绿头野鸭蛋品质研究及蛋壳强度相关基因的筛选

发布时间：2018-04-13 10:27

本文选题：绿头野鸭 + 蛋品质　；参考：《中国畜牧杂志》2017年04期

【摘要】：本试验选取体重、周龄一致的100只绿头野鸭,对其蛋品质进行测定并进行相关分析,测定指标包括蛋壳颜色、蛋壳强度、蛋壳厚度、蛋形指数、蛋白高度、哈氏单位和蛋黄颜色,同时通过转录组测序筛选了与蛋壳强度相关的候选基因。结果表明:不同蛋壳颜色的鸭蛋之间蛋形指数、蛋壳强度、蛋壳厚度、蛋白高度和哈氏单位有显著差异(P0.05);绿头鸭大部分蛋品质性状之间相关性显著,其中蛋壳厚度与蛋壳强度呈显著正相关,相关系数为0.8742,而蛋壳颜色作为蛋壳强度的衡量指标时,白壳组强度最大,绿壳组次之,黄绿壳组最小(P0.01);通过转录组测序对蛋壳强度进行差异表达模式对比分析,共找到差异表达基因325个,其中149个基因表达量上调,176个基因表达量下调,与蛋壳强度相关的基因有CACNA1I、CACNB4、CALN、CACNA1S、LOC101801793等17个基因,这些基因主要参与了钙离子跨膜转运、钙离子结合、钙离子释放等生物学过程,本研究结果为进一步研究蛋壳强度差异的分子机理提供了基础。
[Abstract]:The egg quality of 100 mallard ducks of the same weight and age was measured and analyzed, including eggshell color, eggshell strength, eggshell thickness, egg shape index and protein height.At the same time, the candidate genes related to eggshell strength were screened by transcriptome sequencing.The results showed that there were significant differences in egg shape index, egg shell strength, egg shell thickness, protein height and Hardy unit between duck eggs with different eggshell colors, and the correlation between most egg quality traits of mallard ducks was significant.There was a significant positive correlation between eggshell thickness and eggshell strength, the correlation coefficient was 0.8742. When eggshell color was taken as an index of eggshell strength, the intensity of white shell group was the largest, followed by green shell group.A total of 325 differentially expressed genes, including 149 up-regulated genes and 176 down-regulated genes, were found by comparative analysis of differentially expressed patterns of eggshell intensity by transcriptome sequencing.There are 17 genes related to eggshell strength, such as CACNA1I, CACNB4, CACNA1ShlLOC101801793 and so on. These genes are mainly involved in the biological processes of calcium ion translocation, calcium binding, calcium release and so on.The results provide a basis for further study on the molecular mechanism of eggshell strength difference.
【作者单位】：中国农业科学院北京畜牧兽医研究所;北京市畜牧总站;
【基金】：现代水禽产业技术体系建设专项(CARS-43)
【分类号】：S834

【相似文献】