刺参四个功能基因的表达分析

发布时间：2018-04-13 07:39

本文选题：刺参 + 功能基因　；参考：《大连海洋大学》2016年硕士论文

【摘要】：刺参(Apostichopus japonicus)隶属于棘皮动物门、游走亚门、海参纲、io手目、刺参科,自然分布在西太平洋的中国、韩国、日本沿海,深受这些国家人民的喜爱,目前刺参面临着质量参差不齐等情况。为了研究刺参的生长发育,本实验利用实时定量PCR的方法研究刺参相关候选基因的表达情况。本文中所用的刺参均由养殖场相同池子捕捞后带回实验室在相同的条件下进行养殖,实验选取了四个海参功能基因来验证其表达量与生长的关系,四个基因分别是:腱生蛋白基因、胶原蛋白α2基因、整合蛋白αⅤ基因以及整合蛋白βL基因;并且每隔一个月为一组,共三组,分别取30头海参的八个组织进行实验。实验结果表明,腱生蛋白基因在三个月龄的时间点时,在肠组织、管足组织、呼吸树组织、皮肤组织、体壁组织、体腔液细胞、疣足组织和纵肌组织等八个组织中均有不同程度的表达,但在体腔液细胞和呼吸树组织中表达较高(P0.05)。胶原蛋白α2基因在管足组织、呼吸树组织、体壁组织、疣足组织和纵肌组织中随着刺参月龄的增加其表达量逐渐增加。在所有组织中该基因的表达量在呼吸树组织中最高,然后是在纵肌组织、疣足组织、体壁组织、肠组织和体腔液细胞等组织的表达量逐渐下降。整合蛋白αV基因在纵肌组织、疣足组织和体壁组织中随着月龄的增加其表达量逐渐增加,整合蛋白βL基因在皮肤组织、体壁组织、疣足组织和管足组织中的表达量较低。研究结果为今后深入研究刺参这四个功能基因提供了参考。
[Abstract]:Sea cucumber (Apostichopus japonicus) belonging to the subphylum echinoderm animal door, walk, Holothuroidea, IO, morinaceae, natural distribution in the Western Pacific coast of Japan, South Korea, Chinese, deeply loved the people of these countries, currently facing the sea cucumber quality is uneven situation. In order to study the growth and development of sea cucumber, expression this experiment using real-time quantitative PCR method of sea cucumber related candidate genes used in this paper. The sea cucumber by farms after the same pond fishing back to the laboratory in the same conditions for breeding, we selected four sea cucumber genes to verify the relationship between the expression level and the growth of the four genes are: tendon born in protein gene, collagen alpha 2 integrin alpha v gene, gene and integrin beta L gene; and every month as a group, a total of three groups, eight groups were taken 30 sea cucumber experiment. The experimental results show that the tenascin gene in three months time, in the intestinal tissue, tube foot tissue, respiratory tree tissue, skin, integument, coelomic fluid cells expressed parapodium tissue and longitudinal muscle tissue of eight groups in different degree, but the expression is higher in coelom fluid cells and respiratory tree tissues (P0.05). Collagen alpha 2 gene in tube foot tissue, respiratory tree tissue, body wall, parapodium organization and longitudinal muscle tissues with sea cucumber age increasing its expression gradually increased. In all tissues and the expression of the gene in respiratory tree tissue is the highest, then in the the longitudinal muscle tissue, parapodium, integument tissue, expression of intestinal tissue and coelomic fluid cells decreased gradually. In the longitudinal muscle tissue of integrin alpha V gene, parapodium tissue and integument tissues with the age increasing its expression gradually increased, integrin beta L gene The wall tissue in skin tissue, and expression of parapodium tissues and tissues of the lower tube feet. The research results provide a reference for the future in-depth study of the sea cucumber four functional genes.

【学位授予单位】：大连海洋大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S917.4

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