冷处理后桉树POD活性、同工酶及基因转录变化分析

发布时间：2018-04-13 16:30

本文选题：桉树 + 耐寒性　；参考：《分子植物育种》2017年02期

【摘要】：为了探讨过氧化物酶(Peroxidase,POD)活性、同工酶及基因表达变化与桉树耐寒性间的相关性,选用耐寒性分别为弱、中、强的韦邓桉、邓恩桉和湘邓桉半年苗龄树苗作材料,先26℃培养两周,再5℃冷处理24 h后,剪取叶片检测3种桉树POD活性,同工酶酶谱和4个POD基因的转录变化。韦邓桉冷处理前后的POD活性无显著性差异,但冷处理后,分子量较大的同工酶表达量降低,分子量较小的同工酶表达量升高。冷处理后,邓恩桉POD活性显著降低,各条同工酶活性均有下降;湘邓桉POD活性升高明显,除L4和L5含量略有下降外,其他酶带活性均有增加。设26℃下相同树种的同种基因表达量为1,冷处理后,韦邓桉的POD1和POD2的表达略有下降,POD3和POD4的表达量分别升高到常温下的1.31和1.92倍;邓恩桉的POD1表达量没有改变,但POD2、POD3和POD4的表达量分别降低到常温下的0.44、0.66和0.66倍;湘邓桉的POD1、POD2、POD3和POD4的表达均有显著降低,分别为常温下的0.79、0.29、0.19和0.20倍。结果表明:冷处理后,POD2、POD3和POD4基因的表达变化与桉树抗寒性呈负相关关系。本研究可为桉树耐寒品种的选育提供参考。
[Abstract]:In order to investigate the peroxidase (Peroxidase, POD) activity, isozyme and gene expression changes and correlation between the cold resistance of Eucalyptus, the cold resistance were weak, strong Wei Deng Deng Enan and Xiang Deng eucalyptus, eucalyptus seedlings seedlings as materials for half a year, the first 26 C were cultured for two weeks and then 5 degrees cold treatment for 24 h after the activity of 3 kinds of Eucalyptus Leaf clipping POD detection, transcriptional changes of Isozyme and 4 POD genes. There was no significant difference before and after the Wei Dengan cold treatment POD activity, but after cold treatment, the expression of Isozyme of higher molecular weight decreased, isozyme of smaller molecular weight. The expression level increased after cold treatment. Deng Enan POD activity decreased significantly, the enzyme activity decreased; POD activity increased significantly in Hunan Dunn eucalyptus, except L4 and L5 content decreased slightly, other enzyme activity were increased. The same gene set the same species under 26 DEG C expression was 1, after cold treatment, Wei Deng Eucalyptus PO The expression of D1 and POD2 decreased slightly, expression of POD3 and POD4 were increased to 1.31 and 1.92 times at room temperature; Deng Enan POD1 expression did not change, but the expression of POD2, POD3 and POD4 were reduced to normal temperature 0.44,0.66 and 0.66 times; Xiang Deng Eucalyptus POD1, POD2, expressed in POD3 and POD4 decreased significantly, respectively at room temperature 0.79,0.29,0.19 and 0.20 times. The results showed that: after cold treatment, POD2, POD3 and POD4 gene expression and cold resistance of Eucalyptus was negatively correlated. This research can provide reference for the breeding of cold resistant Eucalyptus varieties.

【作者单位】：岭南师范学院生命科学与技术学院;岭南师范学院资源植物工程中心;
【基金】：国家自然科学基金(31570660) 广东省自然科学基金(2014A030307005;2015A030313560;2016A030313668) 湛江市科研项目(2014A03014)共同资助
【分类号】：S792.39

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