囊泡相关膜蛋白8基因多态性与动脉粥样硬化性脑梗死相关性研究

发布时间：2018-04-13 21:54

本文选题：动脉粥样硬化性脑梗死 + 囊泡相关膜蛋白　；参考：《国际神经病学神经外科学杂志》2017年01期

【摘要】：目的探讨囊泡相关膜蛋白8(VAMP8)基因rs13426038和rs10666612多态性与动脉粥样硬化性脑梗死(ACI)的相关性。方法采用聚合酶链反应-限制性片段长度多态性技术,对动脉粥样硬化性脑梗死组和正常对照组VAMP8基因rs13426038和rs10666612位点进行基因分型。结果两位点基因型分布均符合Hardy-Weinberg遗传平衡检验。VAMP8基因rs13426038位点CC/GG基因型频率在ACI组和对照组分别为20.5%/35.9%和15.0%/36.0%,C/G等位基因频率为42.3%/57.7%和39.5%/60.5%;两组间基因型及等位基因频率差异比较无统计学意义(P=0.08,P=0.25)。VAMP8基因rs10166612位点AA/GG基因型频率在ACI组和对照组分别为92.7%/0.5%和91.8%/0%,A/G等位基因频率为96.1%/3.9%和95.9%/4.1%;两组间基因型及等位基因频率分布比较亦无统计学差异(P=0.16,P=0.46)。结论 VAMP8基因rs13426038 C/G和rs10166612 A/G多态性可能与ACI发生无关。
[Abstract]:Objective to investigate the association between rs13426038 and rs10666612 polymorphism of vesicular associated membrane protein 8 (VAMP8) gene and ACI in atherosclerotic cerebral infarction.Methods VAMP8 gene rs13426038 and rs10666612 loci were classified by polymerase chain reaction-restriction fragment length polymorphism (PCR) in patients with atherosclerotic cerebral infarction (ACI) and normal controls.Results the frequencies of CC/GG genotypes at rs13426038 locus of VAMP8 gene in ACI group and control group were 20.5 / 35.9% and 15.0 / 36.0% respectively, and the frequencies of C / G allele were 42.33.3% and 39.5% / 60.5%, respectively, and the difference of genotype and allele frequency between the two groups was significant.There was no significant difference in the frequency of AA/GG genotypes at the rs10166612 locus in the ACI group and the control group (92.7 / 0.5% and 91.8% vs 91.8%), respectively. The frequencies of the AP-G allele in the ACI group and the control group were 96.1 / 3. 9% and 95. 995 / 1%, respectively, and there was no significant difference between the two groups in the frequency distribution of the genotype and allele. There was no significant difference between the two groups in the frequency distribution of the genotype and allele, and there was no significant difference between the two groups in the frequency distribution of the genotype and allele.Conclusion the polymorphism of rs13426038 C / G and rs10166612 A / G in VAMP8 gene may not be associated with the occurrence of ACI.
【作者单位】：中南大学湘雅医院神经内科;
【分类号】：R743.33

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