稻瘟病菌不同生长期蛋白精氨酸甲基转移酶基因的表达分析

发布时间：2018-04-13 23:10

本文选题：稻瘟病菌 + PRMTs　；参考：《生物技术通报》2017年06期

【摘要】：精氨酸甲基化是一种广泛存在于真核生物中的蛋白质翻译后修饰,其主要过程为蛋白质精氨酸甲基转移酶(Protein arginine methyltransferases,PRMTs)催化S-腺苷甲硫胺酸(S-adenosylmethionine,SAM)提供的甲基基团转移到蛋白质精氨酸侧链胍基基团上。由稻瘟病菌(Magnaporthe oryzae)侵染水稻引起的稻瘟病,每年均会为水稻生产造成严重损失。近年来稻瘟病菌和水稻已成为研究植物病原真菌与寄主植物互作的理想模式生物。生物信息学分析表明,稻瘟病菌中含有4个PRMT基因(Mo PRMT1-4),经蛋白序列比对分析发现,它们均含有保守的甲基转移酶结构域;构建系统发育树,表明稻瘟病菌PRMTs在丝状真菌中高度保守;通过提取稻瘟病菌在不同生长和侵染时期的m RNA,利用实时荧光定量PCR技术,分析稻瘟病菌PRMTs基因的表达谱,发现Mo PRMT1在侵染后24 h表达量达到最高峰,而其他阶段表达水平基本一致;Mo PRMT2与其他3个基因相比,各阶段表达量均较低,且各时期表达水平也没有明显变化;Mo PRMT3在芽管和附着胞发育阶段表达量较高,Mo PRMT3和Mo PRMT4在成熟附着胞时期表达量均达到最高,Mo PRMT4在侵染后42 h也出现表达峰值。这些数据表明PRMTs基因对于稻瘟病菌的侵染致病可能起重要调控作用。
[Abstract]:Arginine methylation is a post-translational modification of proteins widely found in eukaryotes.The main process is that the methyl group provided by S-adenosylmethionine (SAM) catalyzed by protein arginine methyltransferasesl PRMTs is transferred to the side chain guanidine group of the protein arginine by S-adenosylmethionine (SAM), the main process of which is that the methyl group provided by S-adenosylmethionine (SAM) is transferred to the protein arginine side chain guanidine group.The rice blast caused by Magnaporthe oryzae (Magnaporthe oryzae) will cause serious losses to rice production every year.In recent years, rice blast fungus and rice have become ideal model organisms to study the interaction between plant pathogenic fungi and host plants.Bioinformatics analysis showed that four PRMT genes, Mo PRMT1-4, were found in rice blast fungus. By protein sequence alignment analysis, they all contained conserved methyltransferase domain, and phylogenetic tree was constructed.The results showed that PRMTs was highly conserved in filamentous fungi, and the expression profile of PRMTs gene was analyzed by real-time fluorescence quantitative PCR technique.It was found that the expression level of Mo PRMT1 reached its peak at 24 h after infection, while the expression level of Mo PRMT2 in other stages was basically the same as that of the other three genes, and the expression levels of Mo PRMT2 in all stages were lower than those of the other three genes.There was no significant change in the expression level of Mo PRMT3 in each stage. The expression levels of Mo PRMT3 and Mo PRMT4 in bud tubules and appressytes were higher than those in the mature appressytes. The highest expression levels of Mo PRMT4 were also found at 42 h after infection.These data suggest that the PRMTs gene may play an important role in controlling the infection and pathogenicity of rice blast fungus.
【作者单位】：中国农业科学院植物保护研究所;
【基金】：国家自然科学基金项目(31422045)
【分类号】：S435.111.41

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