绵羊Toll样受体7基因多态性分析

发布时间：2018-04-14 14:27

本文选题：Toll样受体 + 多态性　；参考：《江苏农业科学》2017年08期

【摘要】：对新疆萨福克羊Toll样受体7基因单核苷酸进行多态性分析,探索绵羊TLR7基因多态性与抗病力的关系,为家畜抗病育种提供候选分子标记。采集新疆地区种羊场萨福克羊血液600份,提取DNA,设计扩增引物进行聚合酶链式反应-单链构象多态性(PCR-SSCP)检测,对突变位点进行测序分析。结果表明,12对扩增引物中,第2、5、6、7对引物扩增产物具有多态性。其中第2对和第7对引物扩增产物存在错义突变,第5对和第6对引物扩增产物存在同义突变。经SSCP分析,第2对引物扩增产物表现为3种带型,第7对引物有2种带型.经序列比对分析存在A343G、A350G、A1244G的错义突变,氨基酸改变分别是Lys突变为Gln,Asp突变为Ser,Glu突变为Arg,且突变都发生在胞外区亮氨酸重复区内。其中第2对引物的带型AB是位点A343G和A350G同时发生突变。位点A343G的突变频率最高为45.0%,A350G突变频率12.0%,而A1244G突变频率为5.8%。检测的突变可能改变TLR7基因的功能,进而影响其对病源的免疫应答。为进一步的疾病相关的研究奠定基础,以调查这些研究结果在家畜育种方面的用处。
[Abstract]:Single nucleotide polymorphism analysis of Toll like receptor 7 gene in Xinjiang Suffolk sheep was carried out to explore the relationship between sheep TLR7 gene polymorphism and disease resistance and to provide candidate molecular markers for animal breeding for disease resistance.600 blood samples from Suffolk sheep in Xinjiang were collected and DNA was extracted. Polymerase chain reaction-single strand conformation polymorphism (SSCP) was used to detect the mutation sites.The results showed that the amplified products of the second and fifth primer pairs were polymorphic.There were missense mutations in the products of the second and seventh pairs of primers, and synonymous mutations in the products of the fifth and sixth pairs of primers.By SSCP analysis, the products of the second pair of primers showed three bands, and the seventh pair of primers had two bands.Sequence alignment analysis showed that there was a missense mutation of A343GN A350GN A1244G, and the amino acid change was that Lys mutated to Glnn Asp mutation to Seroglu mutation to Arg, and all the mutations occurred in the leucine repeat region of extracellular region.The band type AB of the second pair of primers was the mutation of A343G and A350G at the same time.The highest mutation frequency of A343G was 45.0 and the mutation frequency of A350G was 12.0, while the mutation frequency of A1244G was 5.8.The detected mutations may alter the function of the TLR7 gene and then affect its immune response to the pathogeny.To lay the foundation for further disease-related research to investigate the usefulness of these findings in livestock breeding.
【作者单位】：石河子大学动物科技学院;
【基金】：兵团博士基金(编号:2014BB016) 国家自然科学基金(编号:30960276、31360589) 留学人员科技活动项目(编号:RSLX201304)
【分类号】：S826.2

【相似文献】