中国荷斯坦牛CXCR1基因编码区SNP多态与临床乳房炎和生产寿命的关联分析

发布时间：2018-04-14 15:21

本文选题：中国荷斯坦牛 + CXCR　；参考：《中国农业科学》2017年12期

【摘要】：【目的】探讨中国荷斯坦牛CXCR1基因编码区SNP突变与临床乳房炎和生产寿命的相关性。【方法】根据CXCR1基因编码区序列,利用PCR-直接测序法对低SCS和高SCS样本各20个样本进行SNP筛查,最后对所选择的4个SNP位点利用飞行质谱法对866头中国荷斯坦牛进行检测,同时收集所检测牛只临床乳房炎和生产寿命等信息,利用多因素方差分析法、Logistic回归、Cox生存回归等方法分析以上SNP位点突变与临床乳房炎发生次数和生产寿命的相关性。【结果】CXCR1基因编码区共发现13个SNP位点(291 CT、333 CG、337 AG、365 CT、570 AG、642 AG、735 CG、816 AC、819 AG、980 AG、995 AG、1008 CT和1068 AG),分为4个连锁群,随后从每个连锁群中各选择1个SNP位点(642 AG,816AC,980 AG和1068 AG),利用飞行质谱法对大样本中国荷斯坦牛进行检测。4个SNP位点共有9种单倍型,其中单倍型GAGG频率最高(0.3141),而单倍型ACAA频率最低(0.0017)。CXCR1-642与2胎牛患临床乳房炎次数有显著相关(P0.05),AG基因型个体2胎奶牛患临床乳房炎次数显著高于AA基因型(P0.05),CXCR1-816 AA基因型个体3胎奶牛患临床乳房炎次数显著低于AC和CC基因型(P0.05),其它SNP位点与各胎次临床乳房炎发生次数均无显著相关(P0.05)。CXCR1-816与奶牛离群月龄有极显著相关(P0.01),与奶牛生产月龄和离群胎次有显著相关(P0.05),CXCR1-816 AA基因型个体生产月龄、离群月龄和离群胎次均显著高于CC基因型个体(P0.05),而其它SNP位点对生产月龄、离群月龄和离群胎次均无显著相关(P0.05)。Cox生存分析表明:只有CXCR1-816位点与奶牛生存时间有显著相关(P0.05),CXCR1-816 CC基因型个体在各时间段的生存概率均低于AA和AC基因型个体。【结论】CXCR1-816 AC突变与中国荷斯坦牛患临床乳房炎次数和生产寿命有显著相关,在进一步验证其功能后,可用于中国荷斯坦牛生产寿命的分子标记辅助选择。
[Abstract]:[objective] to investigate the relationship between SNP mutation of CXCR1 gene coding region and clinical mastitis and production life in Chinese Holstein cattle. [methods] according to the sequence of CXCR1 gene coding region,PCR- direct sequencing was used to screen 20 samples of low SCS and 20 samples of high SCS, and 4 SNP loci were selected to detect 866 Chinese Holstein cattle by flight mass spectrometry (FMS).At the same time, we collected the information of clinical mastitis and life span of production.Multivariate variance analysis and logistic regression were used to analyze the correlation between the mutation of SNP locus and the frequency of clinical mastitis and the life span of clinical mastitis. [results] A total of 13 SNP loci were found in the coding region of CXCR1 gene.CG337AGN365CTT 570AGCU 642 AGC735CG716AC819 AGC980AG995 AGC1008CT and 1068 AGNs are divided into 4 linkage groups.Then, one SNP locus was selected from each linkage group to detect large sample Chinese Holstein cattle by flying mass spectrometry. There were 9 haplotypes at the four SNP loci.The frequency of haplotype GAGG was the highest (0.3141g), while the frequency of haplotype ACAA was the lowest (0.0017). CXCR1-642 had a significant correlation with the frequency of clinical mastitis in two cows with P0.05AG genotype. The frequency of clinical mastitis in two pregnant cows was significantly higher than that of AA genotype (P0.05CXCR1-816AA).The frequency of clinical mastitis was significantly lower than that of AC and CC genotypes (P0.05). There was no significant correlation between other SNP loci and the frequency of clinical mastitis in each birth order. CXCR1-816 had a significant correlation with the age of dairy cows at different months of age, and had a significant correlation with the production age of dairy cows.There was a significant correlation between CXCR1-816AA genotype and outlier birth order, and the age of the individuals with CXCR1-816AA genotype was significant.The age of outliers and the number of outliers were significantly higher than those of CC genotype individuals (P 0.05), while the other SNP loci were significantly higher than those of CC genotypes.The survival rate of CXCR1-816CC genotype was significantly lower than that of AA and AC genotypes. [Conclusion the mutation of CXCR1-816 AC was significantly correlated with the number of clinical mastitis and life span of Chinese Holstein cattle.After further verification of its function, it can be used for molecular marker assisted selection of production life of Chinese Holstein cattle.
【作者单位】：扬州大学动物科学与技术学院;江苏君乐宝乳业有限公司;
【基金】：国家自然科学基金(31372286) 江苏省优势学科(PAPD) 江苏省企业研究生工作站资助
【分类号】：S823

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