青花菜肉桂酰辅酶A还原酶基因的克隆与表达特征分析

发布时间：2018-04-14 15:42

本文选题：青花菜 + 肉桂酰辅酶A还原酶　；参考：《热带作物学报》2016年11期

【摘要】：为研究肉桂酰辅酶A还原酶在青花菜生长发育中的作用,采用RT-PCR技术克隆青花菜Bo CCR基因全长c DNA序列,并利用q RT-PCR检测其在不同器官中的表达模式。结果表明:Bo CCR基因开放阅读框为987 bp,推导编码328个氨基酸。预测蛋白分子量为36.86 ku,PI值为6.04。该蛋白亲水性氨基酸多于疏水性氨基酸,为亲水性蛋白。高级结构预测表明青花菜Bo CCR蛋白具有完整的二级结构,三级结构的α-螺旋主要位于外部,β-折叠位于内部。分子进化分析显示CCR蛋白在植物进化过程中,各属形成单独的分枝,可以区分其亲缘关系。荧光定量技术检测到青花菜的Bo CCR基因在花蕾发育早期表达最高,推测该基因可能与青花菜花粉发育相关。
[Abstract]:In order to study the role of cinnamyl coenzyme A reductase in the growth and development of broccoli, the full-length c DNA sequence of CCR gene of broccoli was cloned by RT-PCR, and its expression patterns in different organs were detected by Q RT-PCR.The results showed that the open reading frame of the CCR gene was 987 BP, encoding 328 amino acids.The molecular weight of the predicted protein is 36.86 Kui Pi value is 6.04.The hydrophilic amino acids are more than hydrophobic amino acids and are hydrophilic proteins.The advanced structure prediction showed that the Bo CCR protein of broccoli had a complete secondary structure, the 伪 -helix of the tertiary structure was mainly located outside, and the 尾 -fold was located in the interior.Molecular evolution analysis showed that CCR protein in plant evolution process, each genus formed a separate branch, can distinguish their kinship.The expression of Bo CCR gene in broccoli was the highest in the early stage of flower bud development, which suggested that the gene might be related to the pollen development of broccoli.
【作者单位】：山西林业职业技术学院园艺系;温州科技职业学院;
【基金】：浙江省重点科技创新团队项目(No.2013TD05) 温州市种子种苗科技创新专项项目(No.N20150002) 浙江省自然科学基金(No.LY12C15009) 浙江省农业新品种选育重大科技专项(No.2012C12903-3-3) 山西林业职业技术学院科研项目(No.20160403)
【分类号】：S635.3

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