豫南香稻品种Badh2基因功能标记的检测及应用

发布时间：2018-04-15 01:10

本文选题：香稻 + Badh基因　；参考：《西南农业学报》2017年08期

【摘要】：【目的】香稻中香味的产生是由于水稻第8染色体上面Badh2基因的功能缺失而导致2-乙酰-1-吡咯啉(2AP)前体物质的增多,进而2AP积累使稻米产生香气。【方法】本研究利用Badh2基因开发的7个功能标记,针对豫南地区16份香稻品种进行了检测与分析。【结果】4份香稻品种属于第2外显子突变类型,2份香稻品种属于第4~5外显子突变类型,5份香稻品种属于第7外显子突变类型;并没有发现Badh2基因启动子区及第12~14外显子突变类型的香稻品种。【结论】通过Badh2基因功能标记的检测与分析,本研究鉴定了豫南香稻品种对应的突变类型,为利用分子标记辅助选择培育优质香稻新品种奠定基础。
[Abstract]:[objective] the production of aroma in aromatic rice is due to the loss of the function of Badh2 gene on chromosome 8 of rice, resulting in the increase of 2-acetyl-1-pyrrolidine 2APs.The accumulation of 2AP produced aroma in rice. [methods] Seven functional markers developed by Badh2 gene were used in this study.16 fragrant rice varieties in southern Henan were detected and analyzed. [results] 4 fragrant rice varieties belong to exon 2 mutation type and 2 fragrant rice varieties belong to exon 4Q5 mutation type and 5 fragrant rice varieties belong to exon 7 mutation type.There were no germplasm of Badh2 promoter region and exon 12C14 mutation type. [conclusion] by detecting and analyzing the functional markers of Badh2 gene, this study identified the corresponding mutation types of fragrant rice varieties in southern Henan Province.It lays a foundation for the cultivation of new varieties of high quality aromatic rice by molecular marker assisted selection.
【作者单位】：信阳师范学院生命科学学院;信阳师范学院大别山农业生物资源保护与利用研究院;信阳市农业科学院;
【基金】：河南省重点科技攻关项目(152102110100;152102110036) 国家自然科学基金(U1604110,31600992;U1404319;31270727) 河南省高等学校重点科研项目(15A180059) 河南省重大科技专项(121100110200) 信阳师范学院高层次人才科研启动基金项目(0201430);信阳师范学院“南湖学者奖励计划”青年项目(2016056) 河南省高校科技创新团队项目(14IRTSTHN012) 大别山农业生物研究院开放课题
【分类号】：S511

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