类番茄茄和多毛番茄CBF1基因转化烟草的表达调控分析

发布时间：2018-04-15 09:18

本文选题：CBF + 类番茄茄　；参考：《华中农业大学学报》2017年06期

【摘要】：利用农杆菌介导的叶盘法将类番茄茄(Solanum lycopersicoides)和多毛番茄(Lycopersicon habrochaites)的CBF1基因转入普通烟草(Nicotiana tabacum L.),通过分析转基因烟草在低温胁迫条件下目的基因的表达水平并结合转录组数据分析差异表达的基因,揭示CBF1基因在烟草中发挥抗冷作用的机制与相关性。结果显示:低温胁迫下,转基因烟草的抗低温能力高于非转基因的野生型;SlCBF1和LhCBF1基因在普通烟草中过量表达后,转录组测序分析表明SlCBF1基因转化的烟草植株与野生型烟草植株相比,差异表达基因为53个,其中33个表达上调,20个表达下调;LhCBF1基因转化的烟草和野生型烟草植株相比,差异表达的基因为106个,表达上调的有62个,表达下调的有44个。表明在烟草中过表达SlCBF1和LhCBF1会影响转基因烟草中受其调控的下游基因的表达水平,进而提高烟草的抗冷能力。
[Abstract]:The CBF1 gene of Solanum lycopersicoides-like and Lycopersicon habrochaites) was transferred to Nicotiana tabacum L. by Agrobacterium tumefaciens mediated leaf disk method. The expression level of the target gene in transgenic tobacco under low temperature stress was analyzed and combined with transcriptome.Data analysis of differentially expressed genes,To reveal the mechanism and correlation of cold resistance of CBF1 gene in tobacco.The results showed that the ability of transgenic tobacco to resist low temperature was higher than that of non-transgenic wild type slCBF1 and LhCBF1 genes in common tobacco under low temperature stress.Transcriptome sequencing analysis showed that there were 53 differentially expressed genes in tobacco plants transformed with SlCBF1 gene, 33 of which were up-regulated, 20 tobacco plants with down-regulated expression of LhCBF1 gene were compared with wild-type tobacco plants.There were 106 differentially expressed genes, 62 up-regulated genes and 44 down-regulated genes.The results showed that overexpression of SlCBF1 and LhCBF1 in tobacco could affect the expression level of downstream genes regulated by SlCBF1 in transgenic tobacco, and then improve the ability of cold resistance of transgenic tobacco.
【作者单位】：东北林业大学林学院;东北农业大学生命科学学院;
【基金】：国家自然科学基金项目(J1210069) 黑龙江杰出青年自然科学基金项目(jc2015004) 北美与中国树种健康调查项目(加拿大合作项目201104069)
【分类号】：Q943.2

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