陆地棉GST基因家族全基因组分析

发布时间：2018-04-15 11:33

本文选题：陆地棉 + 谷胱甘肽转移酶　；参考：《遗传》2017年08期

【摘要】：谷胱甘肽转移酶(glutathione-S-transferase,GST)是一种普遍存在的具有多功能的超家族蛋白,在植物初次生代谢、逆境胁迫、胞间信号传递等方面具有重要作用;同时,作为配体其在植物激素代谢以及物质转运方面也发挥作用。为了解析陆地棉(Gossypium hirsutum L.)GST基因家族的信息,本研究对该基因家族成员的种类、进化关系、物理定位、基因结构和保守基序以及表达模式进行了分析。结果显示,在陆地棉全基因组中共含有70个GST基因,进化树和基因结构分析将该家族分为U族、F族、T族、Z族、EF1Bγ族和TCHQD族。基因定位分析发现,除了AD/At2、AD/At4、AD/At5、AD/Dt5、AD/Dt10号染色体上没有GST基因外,其他染色体上都有GST基因,并且在AD/At9、AD/Dt7、AD/Dt12、AD/Dt13这4条染色体上出现基因簇。对F族(Phi类)9个GST基因进行荧光定量分析,结果表明,除Gh GSTF1可能为假基因外,Gh GSTF2~9等8个基因在陆地棉根、茎、叶以及各个发育时期的纤维中均有表达;结合生物信息学分析,推测Gh GSTF8可能参与原花青素/花青素的转运和积累;Gh GSTF4、Gh GSTF6和Gh GSTF9可能在调节陆地棉的生长和胁迫反应中起作用,而Gh GSTF2、Gh GSTF3、Gh GSTF5和Gh GSTF7的功能还有待进一步研究。本研究为陆地棉GST基因家族的分子进化及功能研究提供了理论依据。
[Abstract]:Glutathione-S-transferase (GST) is a multifunctional superfamily protein, which plays an important role in plant primary metabolism, stress stress and intercellular signal transduction.As ligands, they also play a role in plant hormone metabolism and substance transport.In order to analyze the information of Gossypium hirsutum L.)GST gene family, the species, evolutionary relationship, physical location, gene structure, conserved motif and expression pattern of Gossypium hirsutum L.)GST gene family were analyzed in this study.The results showed that there were 70 GST genes in the whole genome of Upland cotton. The phylogenetic tree and gene structure analysis showed that the family was divided into U family, F family, T group, T family, group B, EF1B 纬 and TCHQD family.Gene mapping analysis showed that there was no GST gene on AD / At2 / AD / At4 / AD5 / Dt5 / AD5 / Dt5 / Dt10, and GST gene was found on all the other chromosomes, and there were gene clusters on the four chromosomes of AD / At9AD7 / Dt7 / AD12 / Dt13.The fluorescence quantitative analysis of 9 GST genes of Group F (Phi) showed that eight genes, including Gh GSTF2~9 and Gh GSTF2~9, were expressed in the roots, stems, leaves and fibers of Upland cotton with the possible pseudogenes of Gh GSTF1, and were analyzed by bioinformatics.It was suggested that Gh GSTF8 might be involved in the transport and accumulation of proanthocyanidins / anthocyanins. Gh GSTF6 and Gh GSTF9 might play an important role in regulating the growth and stress response of upland cotton, while the functions of Gh GSTF2 + Gh GSTF3Gh GSTF5 and Gh GSTF7 need to be further studied.This study provides a theoretical basis for the molecular evolution and functional study of GST gene family in upland cotton.
【作者单位】：安徽农业大学生命科学学院;
【基金】：国家自然科学基金项目(编号:31672497) 安徽省“115”产业创新团队项目(皖人才办[2011]2号)资助~~
【分类号】：Q943.2;S562

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