转异苞滨藜BADH基因玉米高世代株系苗期外源基因表达及耐盐性功能分析

发布时间：2018-04-16 08:19

  本文选题：玉米 + BADH基因　； 参考：《玉米科学》2016年03期

【摘要】：以转来自耐盐植物异苞滨藜的甜菜碱醛脱氢酶基因(BADH)玉米T8代株系BZ-136及受体对照自交系郑58(耐盐)为试材,采用盆栽种植方法,分析转基因植株中外源基因的表达情况,检测耐盐相关生理指标。结果表明,转基因株系BZ-136中的BADH酶活性及甜菜碱含量显著高于受体对照,随着盐胁迫时间的延长,其表达量先增加后减少,在胁迫7 d时分别达到了最大值。转基因株系幼苗株高和干重从盐胁迫第3天开始显著高于对照,鲜重和含水量在胁迫第5天和第7天时转基因株系显著高于对照;转基因株系根总体积显著高于对照,直径和根尖数在胁迫第5天时显著高于对照;转基因株系电导率和丙二醛含量均低于对照,分别在胁迫第3天和第7天开始达显著差异,叶绿素含量高于受体对照,从第3天开始二者差异达显著水平。由于外源BADH基因的表达显著提高了基因株系的耐盐性。
[Abstract]:The transgenic maize T8 strain BZ-136 and the receptor inbred line Zheng 58 (salt tolerance) were used to analyze the expression of foreign genes in transgenic plants by pot planting.The physiological indexes related to salt tolerance were detected.The results showed that the activity of BADH enzyme and the content of betaine in BZ-136 were significantly higher than those in the receptor control. With the prolongation of salt stress time, the expression of BADH increased first and then decreased, and reached the maximum value at 7 days of stress.The plant height and dry weight of transgenic lines were significantly higher than that of the control from the third day of salt stress, the fresh weight and water content were significantly higher than that of the control on the 5th and 7th day of stress, the total root volume of the transgenic lines was significantly higher than that of the control, and the total root volume of the transgenic lines was significantly higher than that of the control.The diameter and the number of root tips were significantly higher than that of the control on the 5th day, the electrical conductivity and malondialdehyde content of the transgenic lines were lower than those of the control, and the chlorophyll content was higher than that of the receptor control at the 3rd and 7th day of stress, respectively.From the third day, the difference between the two reached a significant level.The expression of exogenous BADH gene significantly improved the salt tolerance of the lines.
【作者单位】： 东北农业大学农学院;
【基金】：转基因重大专项“转基因玉米小麦大豆环境安全评价技术”(2015ZX08011003) 东北农业大学科学研究基金项目(2012RCB21)
【分类号】：S513
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本文编号：1758069