含氟高分子基因载体

发布时间：2018-04-16 22:21

本文选题：含氟高分子 + 基因递送　；参考：《高分子学报》2017年08期

【摘要】：阳离子高分子被广泛应用为非病毒类基因载体,但这类高分子材料的转染效率与细胞毒性之间通常存在"恶性"关联,即获得高转染效率时往往会伴随严重的细胞毒性.如何制备兼具高效、低毒特点的高分子载体是成功实施基因治疗的关键.含氟高分子是一类具有独特理化性质的高分子,能够在低电荷密度条件下与核酸形成稳定的复合物,从而实现高效、低毒的基因转染.含氟功能基团可帮助阳离子高分子改善复合物稳定性、细胞内吞、内涵体逃逸、胞内核酸释放等多个环节,从而赋予了含氟高分子在基因递送过程中的氟效应.该专论系统地总结了含氟高分子基因载体的研究,介绍了含氟高分子的基因递送性能、作用机理以及在基因治疗、基因编辑中的应用,并对含氟高分子载体的未来发展进行了展望.
[Abstract]:Cationic polymers are widely used as non-viral gene vectors, but there is usually a "malignant" correlation between transfection efficiency and cytotoxicity of such polymer materials, that is, high transfection efficiency is often accompanied by severe cytotoxicity.How to prepare polymer carriers with high efficiency and low toxicity is the key to successful gene therapy.Fluorine-containing polymers are a class of polymers with unique physical and chemical properties, which can form stable complexes with nucleic acids at low charge density, and thus achieve high efficiency and low toxicity gene transfection.Fluorine-containing functional groups can help cationic polymers to improve the stability of the complex, endocytosis, escape of connotations and release of nucleic acid in the cell, thus giving fluorine effect of fluorine-containing polymers in gene delivery process.This monograph summarizes systematically the research of fluorinated polymer gene vector, introduces the gene delivery performance, action mechanism and application in gene therapy and gene editing of fluorinated polymer.The future development of fluorine-containing polymer carrier is prospected.
【作者单位】：华东师范大学生命科学学院;
【基金】：国家自然科学基金(基金号21474030,21322405)资助项目
【分类号】：O631;R450

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