伊斯帕汗小麦NAM-B1基因序列与蛋白质含量变异的分析

发布时间：2018-04-16 23:27

本文选题：伊斯帕汗小麦 + NAM-B基因　；参考：《麦类作物学报》2017年03期

【摘要】：野生二粒小麦(Triticum dicoccoides K錸rn.,AABB,2n=4x=28)NAM-B1基因的表达可以加速植株衰老,促进叶片营养向籽粒转移,提高籽粒蛋白质、铁、锌等物质含量。本研究采用同源克隆方法从6份伊斯帕汗小麦(T.ispahanicum Heslot,AABB,2n=4x=28)中克隆得到5个NAM-B1基因。它们具有典型的NAM基因核苷酸结构特点,均含有3个外显子和2个内含子。与已报道的NAM-B1基因比对分析发现,居群PI330548中的NAM-B1基因因核苷酸序列上第11位点T的插入而引起移码突变,具有无功能型基因的结构特征,其他4个则具有功能类型基因的结构特征。4个功能类型NAM-B1基因的推导氨基酸序列一致性高达99.7%,它们之间仅有5个氨基酸的变异。供试的6份伊斯帕汗小麦之间籽粒蛋白质含量(GPC)存在显著差异。与高GPC材料PI346782相比,低GPC材料PI572904的NAM-B1基因编码氨基酸序列中存在2个位点的变异,分别为NAC域内的C亚区第88位Q→R和TAR区域第364位P→R的替换。推测伊斯帕汗小麦GPC的变异与其NAM-B1基因的这些突变存在一定关系。
[Abstract]:The expression of NAM-B1 gene in Triticum dicoccoides K rhenium rn. AABB _ 2N ~ (2 +) N ~ (2 +) N ~ (2 +) ~ (28) N ~ (2 +) can accelerate plant senescence, promote leaf nutrient transfer to grain, and increase grain protein, iron, zinc and other substances.In this study, five NAM-B1 genes were cloned from 6 Espa Khan wheat (T. ispahanicum Heslotlota, AABB _ (2) N ~ (4) x) by homologous cloning method.They have typical nucleotide structure of NAM gene and contain 3 exons and 2 introns.Comparing with the reported NAM-B1 gene, it was found that the NAM-B1 gene in the population PI330548 was caused by the insertion of T at site 11 on the nucleotide sequence, resulting in a frameshift mutation, which had the structural characteristics of a non-functional gene.The deduced amino acid sequences of the four functional types of NAM-B1 genes were 99.7 consistent, and there were only 5 amino acid variations between them.There was a significant difference in grain protein content (GPC) among 6 Espa Khan wheat lines.Compared with the high GPC material PI346782, there were two mutations in the amino acid sequence of the NAM-B1 gene of the low GPC material PI572904, which were the replacement of the 88th QR of the C subregion and the 364th PERR of the TAR region in the NAC domain.It is speculated that the variation of GPC in Espa Khan wheat is related to the mutation of its NAM-B1 gene.
【作者单位】：四川农业大学小麦研究所;河南科技学院小麦中心;
【基金】：教育部高等学校博士学科点专项科研基金(博导类)项目(20125103110003) 四川省科技厅国际合作项目(2015HH0021) 河南科技学院引进高层次人才科研启动项目(201010613001)
【分类号】：S512.1

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