BMPR1B基因在细毛羊皮肤毛囊中的表达及与羊毛性状关系的研究

发布时间：2018-04-17 00:14

本文选题：细毛羊 + 皮肤　；参考：《中国畜牧杂志》2017年01期

【摘要】：本研究旨在探讨BMPR1B基因在皮肤毛囊的表达及与羊毛性状的关系,为解析其对羊毛相关性状的调控机理奠定理论基础。以敖汉细毛羊为研究材料,分别采集肩部(多毛区)和腹股沟部(少毛区)皮肤组织样。通过基因芯片技术,分析BMPR1B基因在皮肤不同部位表达量的差异;应用实时荧光定量PCR技术,检测BMPR1B基因在细毛羊肩部的表达量,并与羊毛性状和毛囊密度进行关联性分析;应用免疫组化方法,定位BMPR1B基因在细毛羊毛囊中表达的位置。结果表明:BMPR1B基因在肩部和腹股沟部均有表达,且在肩部表达量较高,差异倍数在2倍以上(P0.05);关联分析显示,BMPR1B基因的m RNA表达量与羊毛细度呈显著正相关,相关系数为0.382(P0.05);免疫组化结果显示,BMPR1B基因在细毛羊皮肤中,主要表达于表皮上皮细胞和内根鞘细胞中。综上,可推测在内根鞘中表达的BMPR1B基因,可能对羊毛细度具有调控作用,这可对细毛羊的育种实践提供理论参考。
[Abstract]:The purpose of this study was to investigate the expression of BMPR1B gene in the hair follicles of the skin and wool traits, which lays the theoretical foundation for the analysis of the regulation mechanism of wool traits. In Aohan Merino sheep as research material, the shoulders were collected (hairy area) and groin (less hair area) of skin tissue. By gene chip technology. Analysis of the difference of expression level of BMPR1B gene in different parts of the skin; the application of real-time fluorescence quantitative PCR technology, detect the expression of BMPR1B gene in sheep shoulder, and correlation analysis with wool traits and hair follicle density; immunohistochemical method, the expression location of BMPR1B gene in wool bursa position. The results showed that the expression of the BMPR1B gene in the shoulder and groin were, and the expression level was high in the shoulder, the difference ratio of more than 2 times (P0.05); correlation analysis showed that BMPR1B gene m RNA expression quantity and fineness of wool was A significant positive correlation, the correlation coefficient was 0.382 (P0.05); immunohistochemistry results showed that BMPR1B gene in sheep skin, mainly expressed in epithelial cells of the epidermis and inner root sheath cells. In conclusion, that the expression of BMPR1B gene in the inner root sheath, regulate the fineness of wool, the breeding practice of sheep provide a theoretical reference.

【作者单位】：青岛农业大学动物科技学院;青岛畜牧兽医研究所;
【基金】：国家绒毛用羊产业技术体系资助项目(CARS-40-04) 青岛农业大学高层次人才科研基金(631410)
【分类号】：S826

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