饲料浮萍水平对大正三色锦鲤TYR及MC1R基因表达的影响

发布时间：2018-04-17 04:02

本文选题：浮萍 + 大正三色锦鲤　；参考：《水产科学》2017年02期

【摘要】：为探究饲料中不同浮萍添加水平对大正三色锦鲤酪氨酸酶TYR以及黑素皮质素受体MC1R基因表达的影响,配制浮萍添加水平分别为0%、3%、6%、9%、13%、14%6种等蛋白(38.6%)饲料饲养大正三色锦鲤(42.19±1.32)g 8周,分别记为Diet 1(0/13)、Diet 2(3/10)、Diet 3(6/7)、Diet 4(9/4)、Diet 5(13/1)、Diet 6(14/0)。每种饲料设3个重复,每个重复饲喂10尾鱼。试验结果表明:(1)TYR基因在大正三色锦鲤的心脏、肝脏、眼睛、黑色皮肤、脑中均有表达,并且在脑、皮肤、眼睛中表达量相对较高;(2)各组织中TYR mRNA表达量随浮萍添加水平的升高均有一定波动,在皮肤与眼睛中,以Diet 6(14/0)组显著高于其他各组(P0.05),在脑中,以Diet 3(6/7)组显著高于除Diet 6的其他各组(P0.05);(3)皮肤与眼睛中MC1R mRNA表达量以Diet 6(14/0)组显著高于其他各组(P0.05),而在脑中各组差异均不显著(P0.05)。综上所述,在本试验条件下,饲料不同浮萍添加水平促进大正三色锦鲤TYR以及MC1R基因表达,有利于鱼体黑色素沉积,因此选择Diet 6(14/0)组饲料投喂大正三色锦鲤最适。
[Abstract]:In order to investigate the effect of different levels of duckweed supplementation on the expression of tyrosinase TYR and melanin receptor MC1R gene in tricolor koi carp, the supplementation level of duckweed was 0 + 3X and 131313146, respectively. The feeding level was 42.19 卤1.32g / g for 8 weeks.It's called Diet 1 / 13 / 13 / 2 / 2 / 10 / 3 / 7 / 7 / 4 / 4 / 4 / 5 / 5 / 6 / 614 / 0 / 0 respectively.Each feed was fed with 3 repeats and 10 fish were fed with each repeats.The results showed that the TYR gene was expressed in the heart, liver, eyes, black skin and brain of the tricolor carp, and was also expressed in the brain and the skin.The expression of TYR mRNA fluctuated with the increase of duckweed supplementation level. In the skin and eyes, the expression of TYR mRNA in the skin and eyes was significantly higher than that in the other groups (P 0.05), and in the brain, the expression of TYR mRNA in the skin and eyes was significantly higher than that in the other groups.The expression of MC1R mRNA in the skin and eyes was significantly higher in the Diet 3 / 6 / 7 group than in the other groups except Diet 6. The expression of MC1R mRNA in the skin and eyes was significantly higher in the Diet 614 / 0 group than in the other groups, but no significant difference was found in the brain.To sum up, under the experimental conditions, different feeding levels of duckweed promoted the expression of TYR and MC1R genes in tricolor koi, which was beneficial to the melanin deposition in fish body. Therefore, it was most suitable to choose the feed of Diet 6 / 14 / 0) group to feed Dazheng tricolor koi.
【作者单位】：天津农学院水产学院天津市水产生态及养殖重点实验室;
【基金】：天津市科技重大专项与工程计划项目(15ZXBFNC00340)
【分类号】：S965.8

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