一种现场快速型HBV基因分型方法及装置

发布时间：2018-04-17 10:31

本文选题：HBV基因分型 + 侧向流试纸　；参考：《中国生物医学工程学报》2017年06期

【摘要】：介绍一种现场快速型HBV(乙型肝炎病毒)基因分型方法及装置,包括生物传感器与手持式荧光检测仪。为了提高检测灵敏度与准确性,采用荧光标记法。荧光检测仪采用简化方法来快速读取侧向流试纸上各测试线及质控线的信号幅值,基于预先建立的HBV基因分型模式匹配模型,实现快速分型。对48份HBV血清样本进行检测,实验结果表明,基于荧光检测仪及侧向流试纸的分型检测结果与传统核酸分析方法的分型检测结果一致,分型准确率达100%。与传统方法相比,现场快速型基因分型方法及装置可降低HBV分型检测的复杂度,缩短检测时间,降低检测成本,对乙肝个体化用药与个性化治疗具有重要意义。
[Abstract]:This paper introduces a fast method and apparatus for HBV genotyping, including biosensor and hand-held fluorescence detector.In order to improve the sensitivity and accuracy of detection, fluorescence labeling method was used.The fluorescence detector uses simplified method to quickly read the signal amplitudes of each test line and quality control line on the lateral flow test paper. The fast typing is realized based on the pre-established HBV genotyping pattern matching model.48 samples of HBV serum were tested. The results showed that the typing results based on fluorescence detector and lateral flow test paper were consistent with those of the traditional nucleic acid analysis method, and the accuracy of typing was 100%.Compared with the traditional methods, the in situ rapid genotyping method and device can reduce the complexity of HBV typing detection, shorten the detection time and reduce the detection cost. It is of great significance for individualized medicine and individualized treatment of hepatitis B.
【作者单位】：北京化工大学信息科学与技术学院;厦门大学公共卫生学院国家传染病诊断试剂与疫苗工程技术研究中心;北京工业大学材料科学与工程学院;北京化工大学北京软物质科学与工程高精尖创新中心;
【基金】：国家自然科学基金(81371711) 北京化工大学微系统与可穿戴医疗设备及生物传感技术创新研究团队(buctylkjcx06) 福建省重点科技项目(2014Y0073)
【分类号】：R512.62

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