携带wcaG毒力基因ST19产酸克雷伯菌致白血病患者死亡的分析

发布时间：2018-04-17 14:25

本文选题：产酸克雷伯菌 + 毒力　；参考：《中国人兽共患病学报》2017年08期

【摘要】：目的了解1例高黏液表型(HMV)产酸克雷伯菌致白血病患者的死亡原因。方法采用Vitek 2Compact全自动微生物仪进行菌株鉴定及药敏试验,黏液丝试验检测HMV表型,PCR方法及基因测序检测主要的高毒力荚膜血清型基因(K1、K2、K5、K20、K54、K57)和毒力基因(rmpA、wcaG、allS、kfu、aerobactin、mrkD、fimH、uge、wabG、cf29a),多位点序列分析(MLST)对该菌株进行分子分型。结果白血病患者血液及肺组织分离株均为产酸克雷伯菌,MLST分型为同一型ST19,该菌株对所测药物均敏感,具有高黏液表型,只检测到wcaG毒力基因,其他毒力基因和所测荚膜血清型基因均阴性。结论携带wcaG毒力基因是ST19产酸克雷伯菌致白血病患者死亡的主要原因,应引起临床关注。
[Abstract]:Objective to investigate the causes of death in a case of leukemia caused by Klebsiella acidogenic bacteria with high mucus phenotype.Methods the strain identification and drug sensitivity test were carried out by Vitek 2Compact automatic microorganism instrument.Detection of HMV phenotype by Myxofilament Test and Gene sequencing Analysis of the main highly virulent serotype gene K1K2OK2K5OK20K20K54K57) and virulence gene rmpAgalus wcagall Skfuall aerobactinia mrkDfimHugeugeugewabGcf29aan (multilocus sequence analysis) were used for molecular typing of the strain.Results the blood and lung tissues of leukemia patients were classified into the same type ST19 by MLST of Klebsiella acidogenic strain. The strain was sensitive to the drugs tested and had high mucus phenotype. Only wcaG virulence gene was detected.All other virulence genes and serotype genes were negative.Conclusion carrying wcaG virulence gene is the main cause of death in leukemia patients caused by Klebsiella ST19.
【作者单位】：南昌大学第二附属医院检验科江西省医学检验重点实验室;
【分类号】：R730.43;R733.7

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