橡胶树生殖发育相关基因的RNA-Seq转录组分析

发布时间：2018-04-17 14:53

本文选题：橡胶树 + 生殖发育　；参考：《分子植物育种》2017年01期

【摘要】：为了揭示橡胶树生殖发育相关基因的表达变化情况,本研究以橡胶树热研7-33-97花序、雄蕊、雌蕊为试验材料,利用Illumina HiSeq~(TM) 2000对早期发育的花序、雄蕊及雌蕊进行RNA-Seq测序,共识别出30 164个长度大于200 bp的Unigenes,与其它物种的序列相似性进行比较,发现所得Unigenes与麻疯树的Unigenes相似数量最多。进一步对测序所得Unigenes进行GO(Gene ontology)功能注释和Pathway显著性富集分析发现这些Unigenes能够编码相应的993种酶(Enzyme,EC),参与了197条生物学通路(pathway),相关Unigenes主要涉及碳水化合物运输与代谢、膜转运代谢以及能量代谢通路,由此推测碳水化合物合成与运输相关基因可能在橡胶树生殖发育过程中起重要调控作用。本研究通过对橡胶树花器官发育相关基因进行转录水平分析,为深入开展橡胶树生殖发育相关功能基因的克隆和功能验证提供了丰富的基因资源,并为揭示橡胶树生殖发育的分子调控机制奠定良好基础。
[Abstract]:In order to reveal the expression of genes related to reproductive development of rubber tree, the rubber tree SCATC 7-33-97 inflorescence, stamen, pistil as experimental materials, the use of Illumina HiSeq~ (TM) 2000 on the early development of the inflorescence, stamen and pistil of RNA-Seq sequencing, consensus don't 30164 length more than 200 bp Unigenes, and sequence other species similarity comparison, found with Unigenes from Jatropha curcas is similar to the Unigenes number of Unigenes. Further sequencing of GO (Gene ontology) functional annotation and Pathway significant enrichment analysis showed that these Unigenes encoding 993 enzymes (Enzyme, EC) corresponding, involved in 197 biological pathways (pathway) Unigenes, mainly involved in carbohydrate transport and metabolism, membrane transport and metabolism and energy metabolism pathway, which indicated that the carbohydrate synthesis and transport related genes may be. To play an important role in the reproductive development of rubber trees. Through the research of rubber tree related to floral organ development in the gene transcription level analysis, to carry out the rubber tree reproductive cloning and functional verification of related genes provides abundant gene resources, and lay a good foundation for revealing the molecular regulation mechanism of rubber tree reproductive development.

【作者单位】：中国热带农业科学院橡胶研究所;海南大学农学院;
【基金】：国家天然橡胶产业技术体系项目(CARS-34) 中国热带农业科学院橡胶研究所基本科研业务费(163002201-6016)共同资助
【分类号】：S794.1;Q943.2

【相似文献】