人食物中毒、屠宰及市售禽肉样品中沙门菌毒力基因结果分析

发布时间：2018-04-18 19:18

本文选题：沙门菌 + 毒力基因　；参考：《中国人兽共患病学报》2017年07期

【摘要】：目的了解石家庄地区不同来源沙门菌毒力基因携带状况,为进一步开展沙门菌风险评估提供基础数据。方法收集石家庄地区2011年至2016年食物中毒、屠宰场鸡肉及市售鸡肉的沙门菌分离株186株,进行血清分型,对8种毒力基因(invA、sopE、agfA、spvR、hilA、stn、pefA、shdA)进行PCR检测。结果石家庄地区不同来源沙门菌中,共检出13种血清型,以肠炎沙门菌为显著优势群,上述8种毒力基因均有检出,其中携带率较高的毒力基因为hilA、stn、invA,分别是90.3%(168/186)、86.6%(161/186)和82.8%(154/186)。结论不同来源沙门菌的毒力基因携带状况不同。生禽食品的终端销售环节出现了较严重的污染,应采取措施加强对石家庄地区各早市流动生禽摊点储存和销售环节的食品卫生监督与防控。
[Abstract]:Objective to investigate the virulence gene carrying status of Salmonella from different sources in Shijiazhuang, and to provide basic data for further risk assessment of Salmonella.Methods 186 strains of salmonella isolated from slaughterhouse chicken and chicken sold on market in Shijiazhuang from 2011 to 2016 were collected for serotyping. PCR was used to detect 8 virulence genes, I. e.Results A total of 13 serotypes were detected in different sources of Salmonella in Shijiazhuang, with Salmonella enteritidis as the dominant group. All of the 8 virulence genes were detected. The virulence group with higher virulence rate was hilAstnn inva, which was 90.38%, 18686.66% 166.66% and 82.8% 154p 1866, respectively.Conclusion the virulence genes of Salmonella from different sources are different.There is serious pollution in the end selling of raw poultry food. Measures should be taken to strengthen the food hygiene supervision and control of the storage and sale of raw poultry stalls in early markets in Shijiazhuang area.
【作者单位】：白求恩国际和平医院传染肝病科;石家庄市疾病预防控制中心;河北机场管理集团有限公司应急中心;
【基金】：河北省卫生计生委2017年度医学科学研究重点课题(No.20170964)~~
【分类号】：R155.3

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