基于CRISPR-Cas9新型基因编辑技术研究

发布时间：2018-04-19 16:31

  本文选题：基因编辑 + CRISPR-Cas　； 参考：《生命科学》2016年08期

【摘要】：高效、特异的目标基因组位点修饰一直是基因工程研究的重点和挑战。靶向基因编辑技术不仅能够有效地用于建立动物和细胞疾病模型、培育动植物新品种,并具有治疗诸多疾病的重大潜力。近年来靶向核酸酶技术的研究取得了重大进展,且逐渐成为基因编辑的主流工具,特别是规律成簇间隔短回文重复序列(CRISPR-Cas9)技术因其靶向编辑目的基因的特异性、高效性和设计的简便性等诸多优点,得到更为广泛的应用。在中国科学院干细胞先导专项的支持下,基因编辑技术攻关团队在进一步改造和应用CRISPR-Cas9技术方面取得了一系列成果,就此进行全方面的总结。
[Abstract]:Efficient and specific target genomic site modification has been the focus and challenge of genetic engineering.Target gene editing technology can be used to establish animal and cell disease models and to cultivate new species of animal and plant effectively, and has great potential for the treatment of many diseases.In recent years, great progress has been made in the study of targeted nuclease technology, and has gradually become the mainstream tool of gene editing, especially the regular clustering short palindrome repeat sequence CRISPR-Cas9, because of the specificity of its target gene editing.High efficiency and design simplicity and many other advantages, has been more widely used.With the support of the stem cell pilot project of the Chinese Academy of Sciences, the key technology team of gene editing has made a series of achievements in the further modification and application of CRISPR-Cas9 technology, which is summarized in all aspects.
【作者单位】： 中国科学院动物研究所干细胞与生殖生物学国家重点实验室;中国科学院上海生命科学研究院生物化学与细胞生物学研究所;
【基金】：中国科学院“干细胞与再生医学研究”战略性先导科技专项(XDA01010409)
【分类号】：Q78
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本文编号：1773859