CEA迷你基因串联体疫苗免疫小鼠脾细胞对CEA阳性肿瘤细胞的杀伤作用及疫苗的安全性评价

发布时间：2018-04-19 21:50

本文选题：癌胚抗原 + 迷你基因　；参考：《中国免疫学杂志》2017年02期

【摘要】：目的:观察CEA迷你基因串联体疫苗pcD NA-triC EA625-667免疫小鼠脾细胞对肿瘤细胞特异性杀伤作用并对疫苗免疫小鼠后的安全性进行评估。方法:BALB/c小鼠随机分为空白载体组(pcD NA3.0)、单倍体疫苗实验组(pcD NACEA625-667)、串联体疫苗实验组(pcD NA-triC EA625-667),肌肉注射法免疫动物,每隔10 d免疫1次,共免疫4次,记录免疫小鼠的体重变化、存活情况以及检测血清ALT、肌酐水平。以疫苗免疫小鼠的脾细胞为效应细胞,以LDH释放法检测其对CEA阳性的小鼠肝癌细胞株(H22-CEA+)、胃癌细胞株(MFC-CEA+)、结肠癌细胞株(CT26-CEA+)以及CEA阴性小鼠肝癌细胞株(H22-CEA-)的特异性CTL的杀伤活性。结果:两种疫苗对CEA阳性的肝癌、胃癌及结肠癌细胞均具有较强的杀伤活性,与PcDNA3.0空载体组相比,差异有统计学意义(P0.01)。而对CEA阴性肿瘤细胞(H22-CEA-)则几乎无影响。迷你串联体基因疫苗pcD NA-triC EA+625-667对肝癌细胞H22-CEA及胃癌细胞MFC-CEA+的杀伤活性强于单倍体基因疫苗pcD NA-CEA625-667(P0.05)。疫苗免疫对小鼠的存活状态、体重变化及肝肾功能指标均无影响。结论:CEA迷你基因疫苗安全有效,能够诱导肿瘤特异性CTL产生,且三倍体串联体疫苗免疫效果优于单倍体疫苗。
[Abstract]:Aim: to observe the specific killing effect of spleen cells on tumor cells of mice immunized with CEA mini-gene tandem vaccine pcD NA-triC EA625-667 and to evaluate the safety of mice immunized with the vaccine. Methods Twenty BALB / c mice were randomly divided into blank vector group (pcD NA3.0), haploid vaccine test group (pcD NACEA625-667) and tandem vaccine test group (pcD NA-triC EA625-667G). Mice were immunized by intramuscular injection once every 10 days for a total of 4 times. The body weight of mice immunized was recorded. Survival status and serum alt, creatinine levels were measured. The spleen cells of mice immunized with vaccines were used as effector cells. LDH release assay was used to detect the specific CTL activity of CEA positive mouse hepatoma cell line H22-CEA, gastric cancer cell line MFC-CEA, colon cancer cell line CT26-CEA and CEA negative mouse hepatoma cell line H22-CEA-. Results: the two vaccines had strong killing activity against CEA positive hepatoma, gastric cancer and colon cancer cells, and the difference was statistically significant compared with PcDNA3.0 empty vector group. However, H22-CEA had little effect on CEA negative tumor cells. The cytotoxicity of mini-tandem gene vaccine pcD NA-triC EA 625-667 was stronger than that of haploid gene vaccine pcD NA-CEA625-667P0.05. Vaccine immunization had no effect on survival status, body weight change and liver and kidney function of mice. Conclusion the microvaccine is safe and effective and can induce tumor-specific CTL production, and triploid tandem vaccine is more effective than haploid vaccine.
【作者单位】：吉林省人民医院医学诊治实验中心;吉林大学第一医院呼吸内科;
【基金】：吉林省科技厅国际合作项目(20140414043GH);吉林省科技厅中青年科技创新领军人才及团队项目(20140519018JH);吉林省科技厅重点实验室项目(20122113)资助吉林省人社厅省人才开发资金(2016年度)
【分类号】：R730.51

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