河北汉族人群白细胞介素-17血清表达水平及基因多态性与乙型肝炎病毒感染的关系

发布时间：2018-04-20 10:07

本文选题：白细胞介素- + 单核苷酸基因多态性　；参考：《实用医学杂志》2017年02期

【摘要】：目的:探讨白细胞介素-17(IL-17)血清表达水平及基因IL-17-197A/G位点单核苷酸多态性与乙型肝炎病毒(HBV)感染及临床转归之间的关系。方法:以222例慢性HBV感染者作为病例组,以88例自限性HBV感染者为对照组。采用PCR结合荧光探针的体外DNA扩增检测技术,定量检测血清标本中的HBV DNA含量;ELISA试剂盒检测血清IL-17表达水平;采用贝克曼SNP分析仪,检测IL-17-197A/G位点基因型及等位基因分布频率。结果:各实验组IL-17表达水平差异具有统计学意义(F=158.1,r~2=0.61,P0.000 1)。L-17-197A/G位点AA、AG和GG基因型分布频率在各组中的比较,差异具有统计学意义(χ~2=46.20,P0.000 1)。GG基因型在自限性感染组中分布频率最高(78.41%),而在慢性乙肝肝硬化组中分布频率最低(19.05%)。AA基因型在慢性乙型肝炎肝硬化组中分布频率最高(67.72%)。结论:IL-17-197A/G位点AA基因型和A等位基因是河北汉族人群中HBV易感的宿主基因。
[Abstract]:Objective: to investigate the relationship between serum interleukin-17 (IL-17) expression level and single nucleotide polymorphism (SNP) of IL-17-197A/G site in patients with hepatitis B virus (HBV) infection and clinical outcome. Methods: 222 cases of chronic HBV infection were selected as case group and 88 cases of self limited HBV infection as control group. In vitro DNA amplification technique with PCR and fluorescence probe was used to quantitatively detect the HBV DNA content in serum samples and Elisa kit was used to detect the expression of serum IL-17, and Beckman SNP analyzer was used to detect the genotype and allelic frequency of IL-17-197A/G locus. Results: there were significant differences in the expression of IL-17 among the experimental groups. The distribution frequency of AA-AG and GG genotypes at the 1).L-17-197A/G locus AA-AG and GG in each group were statistically significant. The difference was statistically significant (蠂 ~ (2 +) ~ (46.20) P _ (0.000) 1).GG genotype) was the highest in the self-limited infection group (78.41%), but the lowest in the chronic hepatitis B cirrhosis group was 19.05%. AA genotype was the highest (67.72%) in the chronic hepatitis B cirrhosis group. Conclusion the AA genotype and A allele at the 1: 17-197 A / G locus are the host genes of susceptibility to HBV in Hebei Han population.
【作者单位】：承德医学院附属医院消化科;承德医学院病理教研室;

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