高温胁迫对‘黄冠’、‘翠玉’梨耐热生理指标及相关基因表达的影响

发布时间：2018-04-21 19:44

本文选题：梨 + 高温　；参考：《热带亚热带植物学报》2017年01期

【摘要】：为探讨高温胁迫对梨树的影响,对2年生‘黄冠’(Pyrus pyrifolia‘Cuiguan’)、‘翠玉’(P.bretschneideri×P.pyrifolia)盆栽苗胁迫后叶片的抗氧化物质、抗氧化酶、渗透物质和激素的变化进行了测定,利用q RT-PCR分析了叶片中抗性基因的表达。结果表明,在高温胁迫下,梨树叶片的叶绿素含量明显下降,而MDA含量则持续上升;梨叶片中的POD和CAT活性总体呈下降趋势,但‘黄冠’的POD和CAT活性均高于‘翠玉’;PRO含量随胁迫时间延长逐渐增加;ASA含量表现出先上升后下降,且‘黄冠’的ASA含量高于‘翠玉’;IAA和ABA含量都随胁迫时间的延长呈下降趋势,但‘黄冠’的IAA和ABA含量比‘翠玉’高。q RT-PCR结果表明,叶片中相关基因的表达量与对应的ASA、IAA和ABA含量变化趋势基本一致。因此,‘黄冠’比‘翠玉’抗高温性能强,且高温处理第4天为‘黄冠’和‘翠玉’梨的生理变化转折点。
[Abstract]:In order to study the effect of high temperature stress on pear, the changes of antioxidants, antioxidant enzymes, osmotic substances and hormones in leaves of two year old 'Pyrus pyrifolia' Cuiguanhuanjiao', P. bretschneideri 脳 P.pyrifolia 'potted seedlings were determined. Q RT-PCR was used to analyze the expression of resistance genes in leaves. The results showed that under high temperature stress, the chlorophyll content of pear leaves decreased significantly, while the content of MDA increased, and the activities of POD and CAT in pear leaves decreased. However, the activities of POD and CAT of 'Huangguan' were higher than those of 'Cuiyu'. The contents of POD and CAT increased gradually with the prolongation of stress time. The ASA content of 'Huangguan' was higher than that of 'Cuiyu' and the content of ABA decreased with the prolongation of stress time. However, the content of IAA and ABA in 'Huangguan' was higher than that in 'Cuiyu'. Q RT-PCR showed that the content of IAA and ABA in 'Huangguan' was higher than that in 'Cuiyu'. The expression of related genes in leaves was basically consistent with the change trend of IAA and ABA contents in leaves. Therefore, Huangguan was better than Cuiyu in high temperature resistance, and the turning point of physiological changes of 'Huangguan' and 'Cuiyu' pear was on the 4th day of high temperature treatment.
【作者单位】：安徽农业大学园艺学院;
【基金】：国家现代农业产业技术体系建设专项(CARS-29-14) 国家自然科学基金项目(31101519)资助~~
【分类号】：S661.2

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