霍山石斛PMM基因的克隆及其表达分析

发布时间：2018-04-22 08:33

本文选题：霍山石斛 + PMM基因　；参考：《热带作物学报》2017年12期

【摘要】：为研究磷酸甘露糖变位酶基因(PMM)的功能及其在多糖生物合成中的表达调控机制,以霍山石斛原球茎为材料,利用RT-PCR技术,克隆PMM基因,对其cDNA序列和表达情况进行分析。结果显示:霍山石斛PMM基因cDNA序列长949 bp,包含一个747 bp的开放阅读框,共编码248个氨基酸,GenBank中的登录号为KY912084。生物信息学分析表明:该蛋白属于HAD超家族,可能是一种稳定的、不具有信号肽和跨膜结构的亲水蛋白。系统进化树分析表明,霍山石斛PMM与铁皮石斛、海枣、油棕、芦笋亲缘关系较近,处于同一分支。qPCR结果显示:PMM基因在茎中的相对表达量最高;在4种不同药用石斛比较中,鼓槌石斛的相对表达量最高,霍山石斛的表达量最低。本研究结果表明PMM基因可能不仅参与到石斛多糖的合成,还与其它化合物的生物合成相关。
[Abstract]:In order to study the function of mannose phosphate mutase gene (PMM) and its regulation mechanism in polysaccharide biosynthesis, PMM gene was cloned from protocorm of Dendrobium huoshanense by RT-PCR technique, and its cDNA sequence and expression were analyzed. The results showed that the cDNA sequence of PMM gene of Dendrobium huoshanense was 949 BP long and contained a 747 BP open reading frame, which encode 248 amino acids in GenBank and the accession number was KY912084. Bioinformatics analysis showed that the protein belongs to HAD superfamily and may be a stable hydrophilic protein without signal peptide and transmembrane structure. Phylogenetic tree analysis showed that PMM of Dendrobium huoshanense was closely related to Dendrobium candidum, jujube, oil palm and asparagus. The relative expression of Dendrobium officinale was the highest, and that of Huoshan Dendrobium was the lowest. The results suggest that PMM gene may be involved not only in the synthesis of polysaccharides of Dendrobium, but also in the biosynthesis of other compounds.
【作者单位】：福建省农业科学院作物研究所;福建省农业科学院花卉研究中心;福建省特色花卉工程技术研究中心;
【基金】：福建省省属公益类科研院所专项(No.2015R1026-8) 福建省农业科学院科技创新项目(No.2015CX-1);福建省农业科学院作物研究所青年开放基金项目(No.2015QN-4);福建省农业科学院科技创新团队建设(No.2016PI-39)
【分类号】：S567.239

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