‘翠冠’梨大果型芽变的细胞学及相关基因表达研究

发布时间：2018-04-22 14:34

本文选题：‘翠冠’梨 + 大果型芽变　；参考：《果树学报》2017年06期

【摘要】：【目的】对造成大果芽变的原因进行探讨。【方法】以‘翠冠’和表现出大果性状的芽变‘潘庄大翠冠’为材料,利用荧光AFLP分子标记进行基因组DNA序列差异分析;用流式细胞仪检测‘潘庄大翠冠’的染色体倍性,用石蜡切片技术进行细胞学观察,用实时荧光定量PCR检测相关基因表达分析。【结果】AFLP分析证实‘潘庄大翠冠’为‘翠冠’的大果芽变,果实内在品质无明显差异。流式细胞检测结果显示,‘潘庄大翠冠’为二倍体,与‘翠冠’相同。‘潘庄大翠冠’梨的细胞分裂期从盛花期开始一直到花后24 d,较‘翠冠’长4 d,花后28 d时,果肉细胞层数显著多于‘翠冠’。细胞周期蛋白D3(cyclin D3,CYCD3)在‘潘庄大翠冠’中的表达量显著高于‘翠冠’。细胞周期蛋白A2(cyclin A2,CYCA2)、细胞周期蛋白依赖性激酶A1(cyclin-dependent kinase A1,CDKA1)和细胞周期蛋白依赖性激酶B2(cyclin-dependent kinase B2,CDKB2)等基因也有表达差异。【结论】‘潘庄大翠冠’确为‘翠冠’的大果芽变,其果实增大的机制并非染色体加倍,而是果实发育过程的细胞分裂期细胞的活跃增殖导致细胞分裂期的延长。
[Abstract]:[objective] to investigate the causes of granulation. [methods] Genomic DNA sequence difference was analyzed by fluorescence AFLP molecular marker using 'Cuiguan' and 'Panzhuang Dazuiguan' as materials. The chromosome ploidy of 'Panzhuang Daziguan' was detected by flow cytometry, and the cytology was observed by paraffin section technique. The expression of related genes was detected by real-time fluorescence quantitative PCR. [results] AFLP analysis confirmed that 'Panzhuang Dazuiguan' was the bud of 'Cuiguan', and there was no significant difference in the intrinsic quality of the fruit. The results of flow cytometry showed that 'Panzhuang Dazui Guan' was diploid, which was the same as' Cuiguan'. The cell division of 'Panzhuang Da Cuiguan' pear was 4 days longer than that of 'Cuiguan' from full flowering to 24 days after anthesis. At 28 days after anthesis, the number of cell layers in pulp was significantly more than that in 'Cuiguan'. The expression of cyclin D3(cyclin D _ 3 (CYCD _ 3) in 'Panzhuang D3' was significantly higher than that in 'Cuiguan'. There were also differences in the expression of cyclin A2(cyclin A2 (CYCA2), cyclin dependent kinase A1(cyclin-dependent kinase A1 (CDKA1) and cyclin dependent kinase B2(cyclin-dependent kinase B2 (CDKB2). [conclusion] 'Panzhuang Grand Cuiguan' is indeed the granulation of 'Cuiguan'. The mechanism of fruit enlargement is not chromosome doubling, but the prolongation of cell division due to the active proliferation of cells during fruit development.
【作者单位】：浙江大学园艺系·农业部园艺植物生长发育与品质调控重点开放实验室;上海市农业科学院林木果树研究所·上海市设施园艺技术重点实验室;
【基金】：浙江省园艺作物育种先进技术团队项目(2013TD05)
【分类号】：S661.2

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