糯质与非糯谷子Waxy基因exon4～exon9区间RT-PCR分析

发布时间：2018-04-23 01:32

本文选题：糯谷子 + Waxy基因　；参考：《分子植物育种》2017年06期

【摘要】：为了明确糯质和非糯谷子Waxy基因c DNA序列变异,为品质育种提供实验依据,以2份糯质和2份非糯谷子为材料,利用特异RT-PCR和序列比对,研究了Waxy基因exon4~exon9区间c DNA序列SNPs及其氨基酸多态性,结果表明,糯质红粘谷(00000537)与白米粘谷的同源性为99.12%,在第163、第192、第245和第358位点检测到4个SNPs;非糯类型丰田501与赤谷4号的同源性为98.9%,在第139、第189、第282、第445和第451位点有5个SNPs。糯质红粘谷(00000537)与非糯品种赤谷4号相似性为98.23%,红粘谷(00000537)在第139、163、189、192、245、358、445和451位点碱基分别为T、G、T、G、G、A、C和A,赤谷4号在上述位点依次分别为C、A、G、A、A、C、T和G。糯质红粘谷(00000537)与非糯品种丰田501的相似性为98.9%,红粘谷(00000537)在163、192、245、282和358位点分别为G、G、G、A和A,丰田501依次分别为A、A、A、G和C。红粘谷(00000537)和赤谷4号exon4~exon9区间的c DNA序列编码的氨基酸序列中,检测出6个氨基酸变异,和丰田501出现3个氨基酸的变异。
[Abstract]:In order to identify the variation of c DNA sequence of Waxy gene in waxy and non-waxy grains, and to provide experimental basis for quality breeding, two waxy and two non-waxy grains were used as materials, and specific RT-PCR and sequence alignment were used. The polymorphism of SNPs and its amino acid in exon4~exon9 interval c DNA sequence of Waxy gene was studied. Four SNPs were detected at the 163th, 192nd, 245th and 358 sites, while the non-waxy type Toyota 501 had 98.9G homology with Chigu 4, and 5 SNPs at 139th, 189th, 282th, 445th and 45th. The similarity between glutinous red gluten and non-waxy cultivar Chigu 4 was 98.23, and the base pairs at site 139163189192245358445 and 451were respectively TGN and TGN, and Chigu No. 4 was CZAGAAACCYT and GG, respectively. The similarity between the glutinous red glutinous grain (00000537) and the non-waxy cultivar Toyota 501 was 98.9. The similarity between the glutinous rice and the non-waxy cultivar Toyota 501 was 163192245282 and 358, respectively. The similarity between the glutinous red glutinous grain and the non-waxy cultivar Toyota 501 was Geng Gan Gan A and A, and the Toyota 501 was Agna Agna and C respectively at the sites of 163192245282 and 358. Six amino acid variations were detected in the c DNA sequence in the exon4~exon9 region of Chigu 4, and 3 amino acids were detected in Toyota 501.
【作者单位】：内蒙古农业大学农学院;
【基金】：由国家自然科学基金项目(31260328;31560394) 内蒙古自治区自然科学基金项目(2014MS0321) 内蒙古自治区科技计划项目(201502043)共同资助
【分类号】：Q943.2;S515

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