陆地棉抗坏血酸过氧化物酶基因家族全基因组生物信息学分析

发布时间：2018-04-23 05:31

本文选题：陆地棉 + APX基因家族　；参考：《棉花学报》2017年01期

【摘要】：【目的】抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)是过氧化物酶家族中的1类成员,可以清除活性氧。APX家族的生物信息学分析有助于更好地了解该家族基因。【方法】利用生物信息学方法,从中国农业科学院棉花研究所提供的陆地棉序列中鉴定出14条APX基因家族序列,并对这14个成员的染色体定位、亚细胞定位、进化关系、理化性质、信号肽、保守基序以及其编码蛋白的结构特征进行了预测分析,并初步分析了部分基因的表达。【结果】APX基因分布广泛,分散在不同的染色体上;且大部分APX蛋白定位在细胞质,是亲水性脂溶蛋白;有3个保守基序,保守性较强;基因起源和进化比较复杂;二级结构的主要元件为α-螺旋和无规则卷曲。根据表达分析推测,第1亚组和第2亚组的基因在棉纤维发育的不同时期起作用。【结论】这些结果有望为APX家族成员的结构与功能挖掘提供参考。
[Abstract]:[objective] ascorbate peroxidase (APX) is a member of the peroxidase family, which can scavenge reactive oxygen species. The bioinformatics analysis of the APX family contributes to a better understanding of the family gene. [methods] the bioinformatics method is used. Fourteen APX gene family sequences were identified from cotton sequences provided by Cotton Institute of Chinese Academy of Agricultural Sciences, and the chromosomal localization, subcellular localization, evolutionary relationship, physicochemical properties, signal peptides of the 14 members were analyzed. The structural characteristics of conserved motifs and their encoded proteins were predicted, and the expression of some genes was preliminarily analyzed. [results] APX genes were widely distributed on different chromosomes, and most of APX proteins were located in cytoplasm. It has three conserved motifs, and the origin and evolution of genes are complex. The main components of secondary structure are 伪 -helix and irregular curl. According to the expression analysis, the genes of subgroup 1 and subgroup 2 play an important role in the development of cotton fiber. [conclusion] these results may provide references for the structure and function mining of members of APX family.
【作者单位】：河北农业大学农学院/教育部华北作物种质资源研究与利用重点实验室/河北省作物种质资源重点实验室;河北农业大学作物学博士后科研流动站;
【基金】：国家自然科学基金(31401426) 河北省自然科学基金(C2015204060) 河北农业大学作物学科梯队建设基金(TD2016C319)
【分类号】：S562

【参考文献】