茶树GGPS基因家族的克

发布时间：2018-04-23 08:02

本文选题：茶树 + r{牛儿基r{牛儿基焦磷酸合酶　；参考：《茶叶科学》2017年02期

【摘要】：r{牛儿基r{牛儿基焦磷酸合酶(Geranylgeranyl diphosphate synthase,GGPS)是萜类合成途径的结构酶,对植物生长发育具有重要意义。本研究通过RACE和RT-PCR方法克隆得到5条潜在的茶树GGPS序列,分别命名为CsGGPS1-4和CsGGPS9,其中CsGGPS9存在3条等位基因,分别是CsGGPS9-1、CsGGPS9-2和CsGGPS9-3,在系统进化树上与其他基因分成两支。蛋白质序列分析表明,茶树GGPS家族成员都具有polyprenyl_synt结构域,不存在信号肽序列。亚细胞定位预测结果显示,CsGGPS1、CsGGPS2和CsGGPS4定位在叶绿体上,CsGGPS3和CsGGPS9定位在线粒体上。通过Swiss Model进行三维建模,结合"three-floor"模型对茶树GGPS家族成员的功能进行预测,预测结果显示,CsGGPS1、CsGGPS2和CsGGPS4是GGPS;CsGGPS3是异源二聚体形式的r{牛儿基焦磷酸合酶的小亚基;CsGGPS9的催化主产物是碳链数大于30的异戊烯基焦磷酸。q RT-PCR分析表明,CsGGPS1整体表达丰度较低,仅在一芽二叶中表达量稍高;CsGGPS2在茶树各个组织中均有表达,在花中表达量最高,且花发育过程中表达量先上升后下降;CsGGPS3在叶和幼根中的表达量高于花,花发育过程中表达平稳;CsGGPS4在茶树各个组织中表达量数值相近,在花发育过程中表达量变化趋势与CsGGPS2相同;CsGGPS9的表达量在成熟叶中显著低于幼嫩叶片。
[Abstract]:Geranylgeranyl diphosphate synthases (diphosphate synthases) are structural enzymes of terpene synthesis pathway, and play an important role in plant growth and development. In this study, five potential GGPS sequences of tea tree were cloned by RACE and RT-PCR, named CsGGPS1-4 and CsGGPS9, respectively. There were three alleles in CsGGPS9, namely CsGGPS9-1, CsGGPS9-2 and CsGGPS9-3, which were separated from other genes in phylogenetic tree. Protein sequence analysis showed that the members of tea GGPS family all had polyprenyl_synt domain, and there was no signal peptide sequence. The results of subcellular localization prediction showed that CsGPS1, CsGGPS2 and CsGGPS4 were located in chloroplasts and CsGGPS3 and CsGGPS9 were located in mitochondria. The function of tea GGPS family members was predicted by Swiss Model and "three-floor" model. The expression of CsGGPS2 was higher only in one bud and two leaves, and the highest expression was in the flower, and the expression of CsGGPS3 in the leaves and young roots was higher than that in the young root, and the expression of CsGGPS3 in the flower development was higher than that in the flower, and the expression of CsGGPS3 in the leaves and young roots of tea plants was higher than that in the young roots. The expression of CsGGPS4 was similar in different tissues of tea plant during flower development, and the expression of CsGGPS9 in mature leaves was significantly lower than that in young leaves.
【作者单位】：中国农业科学院茶叶研究所国家茶树改良中心农业部茶树生物学与资源利用重点实验室;
【基金】：国家茶叶产业技术项目(CARS-023) 中国农业科学院科技创新工程(CAAS-ASTIP-2014-TRICAAS)
【分类号】：S571.1

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