转cry1Ab和epsps基因玉米C0030.3.5对土壤古菌丰度和多样性的影响

发布时间：2018-04-23 23:17

本文选题：cryAb + epsps　；参考：《农业环境科学学报》2017年10期

【摘要】：为探讨转基因玉米的生态安全问题,2015年分别于玉米拔节期、抽雄期、乳熟期和完熟期采集土壤样品,并采用荧光定量PCR(Quantitative real-time PCR,q PCR)和末端限制性片段长度多态性分析(Terminal restriction fragment length polymorphism,TRFLP)技术,研究了转cry1Ab和epsps基因玉米C0030.3.5种植对土壤古菌丰度和多样性的影响。结果表明:转cry1Ab和epsps基因玉米C0030.3.5(TM)和受体玉米DBN318(PM)的根际土和非根际土古菌数量为1.41×10~9~4.04×10~9copies·g~(-1)土,随生长时期的推进均呈现先升高后降低的变化趋势,同一生长时期和土壤样品采集区域,2种玉米古菌16S rRNA基因丰度间无显著差异。T-RFLP分析共获得15种不同长度的T-RFs,其中89 bp和184 bp片段为优势种群,同一生长时期和土壤样品采集区域每种优势种群在TM和PM间均无显著差异。土壤古菌Shannon指数除PM非根际土呈现先降低后升高的变化趋势外,其他均表现为先降低后升高再降低;TM和PM根际土古菌Evenness指数的变化趋势均为先降低后升高,而非根际土古菌Evenness指数表现为先降低后升高再降低。同一生长时期和土壤样品采集区域TM和PM的Shannon指数间及Evenness指数间差异均不显著。冗余分析(Redundancy analysis,RDA)显示土壤总氮和硝态氮含量对古菌群落结构有显著影响。主成分分析(Principal component analysis,PCA)表明,TM和PM土壤古菌群落在根际土和非根际土中均未发生明显分离,说明TM和PM土壤古菌群落组成无显著差异。上述结果表明:转cry1Ab和epsps基因玉米C0030.3.5土壤古菌丰度和群落结构组成与受体玉米DBN318无显著差异,古菌丰度和多样性主要受生长时期的影响,受土壤样品采集区域的影响不显著,土壤总氮和硝态氮是调控土壤古菌群落变化的关键因子。
[Abstract]:In order to study the ecological safety of transgenic maize, soil samples were collected in jointing, heading, milking and ripening stages in 2015, respectively. The effects of cry1Ab and epsps gene maize (C0030.3.5) on the abundance and diversity of soil paleomycetes were studied by means of fluorescence quantitative PCR(Quantitative real-time PCRQ PCR and terminal restriction fragment length polymorphism (TRFLP) technique. The results showed that the number of archaic bacteria in rhizosphere soil and non-rhizosphere soil of transgenic cry1Ab and epsps gene maize C0030.3.5 and receptor maize DBN318PM) was 1.41 脳 10 ~ (9) ~ (9) 脳 10~9copies ~ (-4) 脳 10~9copies ~ (-1), which increased at first and then decreased with the development of growth period, and the results showed that the number of bacteria in rhizosphere and non-rhizosphere soil was 1.41 脳 10 ~ (9) and 4.04 脳 10~9copies / g ~ (-1), respectively. There was no significant difference in the abundance of 16s rRNA gene between two species of maize palaeomycetes in the same growing period and soil sample collection region. T-RFLP analysis showed that 15 T-RFSs of different lengths were obtained, of which 89bp and 184bp were dominant populations. There was no significant difference between TM and PM in each dominant population in the same growing period and soil sampling area. The Shannon index of soil palaeomycetes decreased first and then increased, except that PM decreased first and then increased, and then decreased and then decreased, and the Evenness index of Palaeobium in PM rhizosphere soil decreased first and then increased. The Evenness index of non-rhizosphere bacteria decreased first and then increased and then decreased. There was no significant difference in Shannon index and Evenness index between TM and PM in the same growing period and soil sample collection area. Redundancy analysis showed that the contents of soil total nitrogen and nitrate nitrogen had a significant effect on the community structure of Palaeomycetes. Principal component analysis (PCA) showed that there was no significant separation of soil paleomycetes community between TM and PM soil in rhizosphere and non-rhizosphere soil, indicating that there was no significant difference between TM and PM soil paleomycetes community composition. The results showed that there was no significant difference between C0030.3.5 soil paleomycetes abundance and community structure composition of transgenic cry1Ab and epsps gene maize and that of recipient maize DBN318. The abundance and diversity of paleomycetes were mainly affected by growth period, but not by soil sample collection area. Soil total nitrogen and nitrate nitrogen are the key factors to regulate the change of soil paleomycetes community.
【作者单位】：农业部环境保护科研监测所;天津农学院农学与资源环境学院;
【基金】：转基因生物新品种培育重大专项(2015ZX08013002-004,2016ZX08012005-005) 国家自然科学基金项目(31200424)~~
【分类号】：S154.3;S513

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