水稻淡黄叶矮化突变体yld的遗传分析及基因定位

发布时间：2018-04-23 23:31

本文选题：水稻 + 淡黄叶矮化突变体　；参考：《作物学报》2017年04期

【摘要】：水稻叶色突变体是研究植物光合作用、叶绿素代谢和叶绿体发育的重要材料。本研究从籼稻品种蜀恢527经EMS(甲基磺酸乙酯)诱变处理后代中筛选出一个淡黄叶矮化突变体Yellow leaf and dwarf(yld)。与野生型蜀恢527相比,该突变体全生育期都表现出淡黄叶矮化性状,其剑叶的淡黄色表型最为明显,倒二叶次之,倒三叶最弱,其中剑叶的叶绿素及类胡萝卜素含量降低最为明显;并且伴随着穗粒数、千粒重、结实率、株高等主要农艺性状的显著降低,但有效穗显著增多。透射电镜观察结果显示,与野生型相比,该突变体多数叶绿体结构基本完整,但基粒模糊,基质片层大量减少且排列疏松。遗传分析表明,该突变性状受一对隐性核基因控制。在yld突变体与粳稻武运粳7号杂交的F2群体中分离出323个突变单株,最终将YLD基因定位在第11染色体的L5和L7两标记之间,物理距离为115.7 kb。本研究为YLD基因的克隆和功能分析奠定了基础。
[Abstract]:Rice leaf color mutant is an important material to study photosynthesis, chlorophyll metabolism and chloroplast development. In this study, a yellowish leaf dwarf mutant Yellow leaf and dwarfffyldfyld1 was selected from the progenies of indica rice cultivar Shuhui 527 treated with EMS- (ethyl methanesulfonate). Compared with the wild type Shuhui 527, the mutant showed the dwarfing character of yellowish leaves in the whole growth period. The yellowish phenotype of the flag leaves was the most obvious, the second leaf was the second leaf, and the third leaf was the weakest. The content of chlorophyll and carotenoid in flag leaves was the most obvious, and accompanied by the number of grains per spike, 1000-grain weight, seed setting rate, plant height and other main agronomic traits, but the effective panicle significantly increased. The results of transmission electron microscopy showed that most of the chloroplasts of the mutant were basically intact, but the granulocyte was vague, the matrix lamellar was decreased and the arrangement was loose. Genetic analysis showed that the mutant trait was controlled by a pair of recessive nuclear genes. A total of 323 mutant plants were isolated from F2 population of japonica rice Wuyunjing 7 and yld mutants. Finally, the YLD gene was located between L5 and L7 markers on chromosome 11 with a physical distance of 115.7 kb. This study laid a foundation for cloning and functional analysis of YLD gene.
【作者单位】：杭州师范大学生命与环境学院;中国水稻研究所水稻生物学国家重点实验室;
【基金】：国家自然科学基金项目(31401464)资助~~
【分类号】：S511

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