贵州省2013年麻疹病毒核蛋白基因特征分析

发布时间：2018-04-24 02:23

本文选题：麻疹病毒 + 核蛋白　；参考：《山东大学学报(医学版)》2017年03期

【摘要】：目的分析2013年贵州省流行的麻疹病毒核蛋白基因特征。方法采集疑似麻疹病例临床标本(咽拭子、尿液和血清),从临床标本或Vero/SLAM细胞分离出的阳性培养物中提取病毒RNA,采用RT-PCR方法扩增麻疹病毒核蛋白基因羧基末端编码的634个核苷酸片段,并进行序列测定与基因特征分析。结果 74例疑似麻疹病例中检测到41株麻疹核酸。基因亲缘关系显示39株为H1基因型中的H1a基因亚型并且有2个明显分支;2株为麻疹疫苗株(A基因型)。39株麻疹病毒株与H1基因型参考株MVi/Hunan.CHN/0.93/7(AF045212)的核苷酸和氨基酸差异分别为2.0%~2.9%和2.0%~4.7%;与H1a基因亚型参考株Chin9322的核苷酸和氨基酸差异分别为0.9%~1.8%和0.7%~3.4%;39株H1a基因亚型的核苷酸和氨基酸同源性分别为97.3%~100%和96.6%~100%。2株麻疹疫苗株与MV沪191(S191)的核苷酸和氨基酸同源性分别为99.8%~100%和99.3%~100%。结论 H1a基因亚型是贵州省2013年本土麻疹流行的优势基因型,同一年份存在着不同的传播链。
[Abstract]:Objective to analyze the nucleoprotein gene characteristics of measles virus in Guizhou province in 2013. Methods the clinical specimens (pharynx swab) of suspected measles cases were collected. In urine and serum, virus RNAs were extracted from clinical specimens or positive cultures isolated from Vero/SLAM cells. 634 nucleotide fragments encoded by carboxyl end of nucleoprotein gene of measles virus were amplified by RT-PCR method. Results 41 strains of measles nucleic acid were detected in 74 suspected measles cases. Genetic relationship showed that 39 strains were H1 genotype H1 gene subtype and 2 strains were measles vaccine strain. 39 strains measles virus strain and H1 genotype reference strain (MViP Hunan. CHN / 0.93% 7% AF045212) had different nucleotide and amino acid differences. The difference of nucleotide and amino acid with Chin9322 was 0.91.8% and 0.710%, respectively. The homology of nucleotide and amino acid between the nucleotide and amino acid of H1 A gene subtype was 97.30.100% and 96.6B / 100.2, respectively. The nucleotide and amino acid homology between measles vaccine strain and MV191A S191 was 97.3ng% and 0.76%, respectively, and the amino acid homology of H1 A gene subtype was 97.31,100.2% and 96.610.2%, respectively, and the amino acid homology was 97.3% and 96.610.2%, respectively. The homology of base acid was 99.8% and 99.3%, respectively. Conclusion H1 a gene subtype is the dominant genotype of measles epidemic in Guizhou province in 2013, and there are different transmission chains in the same year.
【作者单位】：贵州省疾病预防控制中心免疫规划所;贵州医科大学基础医学院免疫学教研室;
【基金】：中国疾病预防控制中心病毒病预防控制所WHO西太平洋区麻疹参比实验室贵州省9个市(州)及2个直管县级麻疹、风疹实验室的支持与帮助
【分类号】：R511.1

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