基于16S rRNA基因部分序列的长江口虾虎鱼科鱼类系统分类

发布时间：2018-04-24 03:01

本文选题：虾虎鱼科 + S　；参考：《海洋渔业》2016年01期

【摘要】：为了确定线粒体16S rRNA基因在长江口虾虎鱼科鱼类系统分类及物种鉴定中的作用,采用16S rRNA基因特异扩增测序及Gen Bank已有序列联合配对分析的方法,对长江口虾虎鱼科9属11种鱼类34个16S rRNA基因片段的序列进行比较和系统分类研究。统计分析显示,虾虎鱼科鱼类该片段的A含量明显高于其它3个碱基含量,A+T的平均含量高于G+C的平均含量,第3密码子位点G+C含量最高,其平均值为51.1%,变化范围为49.8%~53.2%。全部转换位点多于颠换位点,转换/颠换比值为1.45。依据Maximum Composite Likelihood模型,得出11种虾虎鱼科鱼类种间遗传距离平均值为0.151,种内为0.002,种间遗传距离是种内遗传距离的76倍。通过邻接法(Neighbor-joining,NJ)构建系统发育树,显示长江口虾虎鱼科鱼类为明显的单系群,并进一步佐证把传统形态分类中弹涂鱼科的青弹涂鱼(Scartelaos histiophorus)和大弹涂鱼(Boleophthalmus pectinirostris)及鳗虾虎鱼科的拉氏狼牙虾虎鱼(Odontamblyopus lacepedii)和红狼牙虾虎鱼(Odontamblyopus rubicundus)归属于虾虎鱼科的合理性。聚类结果显示红狼牙虾虎鱼和拉氏狼牙虾虎鱼聚在一起,其节点支持率达100%,两者可能为同种异名。本研究表明,线粒体16S rRNA基因序列作为分子标记对虾虎鱼科鱼类进行物种鉴定和系统分类是可行的,可为虾虎鱼类的亲缘关系分析提供基础资料。
[Abstract]:In order to determine the role of mitochondrial 16s rRNA gene in the taxonomy and species identification of Shrimp Tiger fishes in the Yangtze River Estuary, the 16s rRNA gene was amplified and sequenced and the existing Gen Bank sequence was combined with pairing analysis. The sequences of 34 16s rRNA gene fragments from 11 species of 11 species belonging to 9 genera in the Yangtze River Estuary were compared and systematically classified. The statistical analysis showed that the A content of this fragment was significantly higher than that of the other three base groups, the average content of AT was higher than that of GC, the content of GC at codon 3 was the highest, the mean value was 51.1, and the range of variation was 49.8% and 53.2%. All the transition sites were more than those on the other hand, the conversion / transversion ratio was 1.45. According to the Maximum Composite Likelihood model, the average genetic distance between species of 11 species was 0.151 and 0.002, and the genetic distance between species was 76 times of that within species. The phylogenetic tree was constructed by Neighbor-joiningnj), which showed that the fishes in the Yangtze River Estuary were monophyletic. The rationality of the traditional morphological classification of Scartelaos histiophorus) and Boleophthalmus pectinirostris (Odontamblyopus laceipedii) and Odontamblyopus rubicundus (Odontamblyopus rubicundus) was further demonstrated. The clustering results showed that the red wolf shrimp tiger and the Lahlus tigfish got together, and the support rate of the nodes reached 100, which may be the same name. The results showed that it was feasible to identify and classify the species of Shrimp Tigronidae by using mitochondrial 16s rRNA gene sequence as a molecular marker, which could provide basic data for the phylogenetic analysis of Shrimp Tiger.
【作者单位】：南京农业大学无锡渔业学院;中国水产科学研究院东海水产研究所;上海海洋大学水产与生命学院;
【基金】：公益性行业(农业)科研专项(201203065)
【分类号】：S917.4

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