微小RNA-200c靶向抑制EFNA1基因对胃癌细胞增殖、凋亡及侵袭的影响

发布时间：2018-04-24 08:31

本文选题：胃癌 + 微小RNA-c　；参考：《山东医药》2017年09期

【摘要】：目的探讨微小RNA(miR)-200c靶向抑制肝配蛋白A1(EFNA1)基因对胃癌细胞增殖、凋亡及侵袭的影响。方法选取胃癌细胞株SGC7901,随机分为miR-200c模拟物组、模拟物对照组、miR-200c抑制物组、抑制物对照组,分别转染miR-200c模拟物、模拟物对照寡核苷酸、miR-200c抑制物、抑制物对照寡核苷酸,采用CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡,Transwell法检测细胞侵袭。采用双荧光素酶实验验证miR-200c对EFNA1基因的靶向抑制作用。取本院手术切除的胃癌和相应癌旁组织标本48例份,通过免疫组化法检测EFNA1蛋白表达,分析胃癌组织中EFNA1蛋白表达与患者性别、年龄、吸烟、饮酒、病理类型、浸润深度、淋巴结及远处转移、肿瘤部位间的关系。结果与模拟物对照组相比,miR-200c模拟物组胃癌细胞SGC7901增殖活性降低(P0.05),总凋亡率升高(P0.05),侵袭能力降低(P0.05)。miR-200c模拟物组的荧光素酶活性低于模拟物对照组(P0.05),而miR-200c抑制物组的荧光素酶活性高于抑制物对照组(P0.05)。EFNA1蛋白在胃癌组织中表达高于癌旁组织,其高表达与胃癌淋巴结转移有关(P0.05),与其他临床病理特征均不相关(P均0.05)。结论胃癌组织中EFNA1基因呈高表达,miR-200c可通过靶向EFNA1基因抑制胃癌细胞增殖及侵袭,促进凋亡。
[Abstract]:Objective to investigate the effects of small RNA(miR)-200c targeting on the proliferation, apoptosis and invasion of gastric cancer cells. Methods gastric cancer cell line SGC7901 was randomly divided into three groups: miR-200c mimic group, mimic control group and inhibitor control group. MiR-200c mimics were transfected into miR-200c mimics, oligonucleotides were transfected with miR-200c inhibitors, and oligonucleotides were controlled by oligonucleotides. CCK-8 assay was used to detect cell proliferation, and flow cytometry was used to detect cell apoptosis and cell invasion. Double luciferase assay was used to verify the targeted inhibitory effect of miR-200c on EFNA1 gene. The expression of EFNA1 protein was detected by immunohistochemical method in 48 cases of gastric cancer and its adjacent tissues. The expression of EFNA1 protein in gastric cancer tissues and patients' sex, age, smoking, drinking, pathological type, depth of infiltration were analyzed. The relationship between lymph nodes, distant metastasis and tumor sites. Results compared with the control group, the proliferative activity of SGC7901 in gastric cancer cells decreased, the total apoptosis rate increased, and the activity of luciferase in the mimic group was lower than that in the control group, while the fluorescent activity in the miR-200c inhibitor group was lower than that in the control group. The activity of photoenzyme was higher than that of the control group. The expression of P0.05U. EFNA1 protein in gastric cancer tissues was higher than that in paracancerous tissues. Its high expression was related to lymph node metastasis of gastric cancer (P 0.05), but not to other clinicopathological features (P 0.05). Conclusion the overexpression of EFNA1 gene in gastric cancer tissues can inhibit the proliferation and invasion of gastric cancer cells and promote apoptosis by targeting EFNA1 gene.
【作者单位】：广州医科大学附属广州市第一人民医院;
【基金】：国家自然科学基金资助项目(81502040) 广州市卫生和计划生育科技一般引导项目(20161A010009)
【分类号】：R735.2

【相似文献】