茶树磷转运蛋白基因CsPT4的克

发布时间：2018-04-24 12:37

本文选题：茶树 + 磷转运蛋白　；参考：《茶叶科学》2017年05期

【摘要】：茶园中磷肥的利用效率取决于茶树体内与磷元素吸收、转运及生理利用等相关蛋白的协同调控,而磷转运蛋白(Phosphate transporter proteins,Phts)在此过程中起着关键的调控作用。本研究以茶树品种龙井长叶(Camellia sinensis cv.Longjing-changye)为试验材料,采用同源克隆的方法首次克隆获得茶树磷转运蛋白编码基因Cs Pht1:4(Cs PT4)的全长c DNA。该基因全长1 642 bp,开放阅读框(ORF)1 620 bp(Gen Bank登录号:KY132100),编码539个氨基酸。生物信息学分析显示,Cs PT4基因编码蛋白分子量为59.12 k D,理论等电点(p I)为8.51;具有典型的Pht1家族特性:"6-亲水-6"跨膜结构。亚细胞定位结果显示,该蛋白分布于质膜上,与Softberry软件预测结果一致。荧光定量PCR表明:Cs PT4在正常生长的茶树根、茎、嫩叶、老叶中均有表达,在老叶中的表达量较高,在根部表达量最低。低磷处理,根和叶中Cs PT4上调表达水平均先上升后下降;根部Cs PT4表达量48 h内各个时间点均高于叶部。缺磷处理,根和叶中Cs PT4上调表达水平均升高;根部和叶部分别在72 h和48 h达最大值。本研究为茶树响应低磷的分子机制提供了参考。
[Abstract]:The utilization efficiency of phosphorus fertilizer in tea garden depends on the synergistic regulation of phosphorus absorption, transport and physiological utilization in tea plants, and phosphorus transport protein Phosphate transporter proteins play a key role in this process. In this study, Camellia sinensis cv. Longjing-changyeh, a tea plant variety, was used as the experimental material. The full-length c-DNAof the tea plant phosphorous transporter gene Cs Pht1:4(Cs PT4 was obtained by homologous cloning for the first time. The full length of the gene is 1 642 BP, ORF 1 620 bp(Gen Bank accession number: KY132100, encoding 539 amino acids. Bioinformatics analysis showed that the molecular weight of the protein encoded by Cs PT4 gene was 59.12 KD and the theoretical isoelectric point I was 8.51, which had typical Pht1 family characteristics: "6-hydrophilic -6" transmembrane structure. The subcellular localization results showed that the protein was located on the plasma membrane, which was consistent with the predicted results by Softberry software. Fluorescence quantitative PCR showed that the expression of PT4 was found in the roots, stems, young leaves and old leaves of tea trees, but the expression level was higher in the old leaves and the lowest in the roots. The up-regulated expression of Cs PT4 in roots and leaves increased first and then decreased, and the expression of Cs PT4 in roots was higher than that in leaves at every time point within 48 h. The up-regulated expression of Cs PT4 in roots and leaves reached the maximum at 72 h and 48 h, respectively. This study provides a reference for the molecular mechanism of tea tree response to low phosphorus.
【作者单位】：南京农业大学茶叶科学研究所;
【基金】：现代农业产业技术体系建设专项资金(CARS-23) 江苏高校优势学科建设工程资助项目国家自然科学基金(31570691) 江苏省科技支撑计划(BE2009313-1)
【分类号】：Q943.2;S571.1

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