滞育及不同虫态下亚洲玉米螟内参基因的筛选

发布时间：2018-04-24 12:43

本文选题：亚洲玉米螟 + 内参基因　；参考：《环境昆虫学报》2017年03期

【摘要】：筛选出滞育状态下以及不同虫态下亚洲玉米螟Ostrinia furnacalis(Guenée)的实时荧光定量PCR(RT-qPCR)最稳定内参基因,本结果为研究不同虫态及滞育1个月,滞育2个月,滞育3个月和滞育4个月的亚洲玉米螟目的基因表达水平提供参考依据。本文以不同虫态及滞育状态下的亚洲玉米螟为试验材料,应用RT-qPCR技术检测β-actin,18S rRNA,EF1-α和RPS3共4个候选内参基因的表达稳定性水平,结合ge Norm,Normfinder,Best Keeper和Ref Finder软件分析各候选内参基因在不同处理下的表达稳定性。结果表明,在亚洲玉米螟不同虫态中,4个候选内参基因的稳定性大小排序为18S rRNAEF1-αβ-actinRPS3;滞育1个月,滞育2个月,滞育3个月和滞育4个月的亚洲玉米螟,稳定性大小排序为β-actinEF1-α18S rRNARPS3。18S rRNA和β-actin可分别作为不同虫态和滞育状态下的亚洲玉米螟基因表达水平分析试验中的内参基因。
[Abstract]:The real-time fluorescent quantitative PCR (RT-qPCR) internal reference gene of Ostrinia furnacalis (Guen e e) of the Asian corn borer (Guen e e) under diapause and different insect States was screened. The results provided a reference for the study of the target gene expression level of the subdiapause and diapause for 1 months, diapause, diapause, 3 months and diapause for 4 months. The expression stability of 4 candidate internal parameter genes of beta -actin, 18S rRNA, EF1- alpha and RPS3 were detected by RT-qPCR technique in different insect state and diapause state, and the stability of the expression stability of the selected internal reference genes under different treatments were analyzed with GE Norm, Normfinder, Best Keeper and Ref software. The results showed that in the different insect states of Asiatic corn borer, the stability of 4 candidate internal reference genes was 18S rRNAEF1- alpha beta -actinRPS3, diapause for 1 months, diapause for 2 months, diapause for 3 months and diapause for 4 months of Asian corn borer, the order of stability was beta -actinEF1- a 18S rRNARPS3.18S rRNA and beta -actin as different insect state and stagnation, respectively. The internal reference genes in the analysis of gene expression level of Asian corn borer under the condition of breeding.

【作者单位】：沈阳农业大学植物保护学院;抚顺出入境检验检疫局;
【基金】：农业部公益性行业科研专项(201303026) 国家重点研发计划(2016YFD0300704)
【分类号】：S435.132

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