葡萄CIPK基因家族的鉴定表达分析

发布时间：2018-04-24 15:15

本文选题：葡萄 + CIPK家族　；参考：《西北农业学报》2017年11期

【摘要】：以水稻、玉米、拟南芥中已知CIPK基因注册序列为基础,从葡萄基因组数据库中电子克隆出16条CIPK基因。对其理化性质分析表明,除VvCIPK10编码的251个氨基酸数目外,其余氨基酸数目基本稳定为300~470。整个CIPK家族的理论等电点为6~9。基因结构分析表明,VvCIPK01、VvCIPK03、VvCIPK04、VvCIPK08、VvCIPK09、VvCIPK13包含外显子数都大于10;VvCIPK02、VvCIPK05、VvCIPK06、VvCIPK07、VvCIPK10、VvCIPK11、VvCIPK12、VvCIPK14、VvCIPK15、VvCIPK16包含外显子数都小于7。聚类分析表明,CIPK基因被分为4个亚族,且在每一个亚族中都包含葡萄和拟南芥的CIPK基因家族成员,说明它们具有很高的同源性。对CIPK蛋白质的二级结构分析表明,葡萄CIPK基因家族所编码的蛋白质均以α-螺旋和不规则卷曲为主,而β-转角最少。亚细胞定位后发现,VvCIPK基因在细胞质中表达最多。对葡萄CIPK基因家族上游2kb区域顺式作用元件分析表明,VvCIPK13对于ABA和脱水胁迫的响应最为明显,葡萄CIPK基因家族对于MYB转录因子和WRKY转录因子均有响应。荧光定量分析表明,VvCIPK15在根中表达量最多,在茎中表达量最少;在不同处理下该基因表达差异性显著。其中,受PEG、ABA、NaCl诱导后呈明显上调表达,其表达量依次为PEGNaClABA。同时该基因在受到高、低温胁迫时表达量也有明显上调,9个处理中只有在山梨糖中呈下调表达。推测该基因能够参与调控干旱、盐碱、低温等逆境过程。
[Abstract]:Based on the known CIPK gene registration sequences in rice, maize and Arabidopsis thaliana, 16 CIPK genes were cloned from grape genome database. The physicochemical properties showed that the number of amino acids, except for the 251 amino acids encoded by VvCIPK10, was basically stable at 300,470. The theoretical isoelectric point of the whole CIPK family is 6 / 9. The gene structure analysis showed that VvCIPK01 / VvCIPK03 / VvCIPK04 / VvCIPK08 / VvCIPK09 / VvCIPK13 contains more exons than 10vvCIPK02 / VvCIPK05VvCIPK06 / VvCIPK07 VvCIPK10vCIPK11VCIPK12vCIPK14VCIPK15vCIPK16 are less than 7VCIPK10VCIPK11vCIPK12VCIPK14VCIPK15vCIPK16. Cluster analysis showed that the CIPK gene was divided into four subfamilies, and each subfamily contained CIPK family members of grape and Arabidopsis thaliana, indicating that they have high homology. The analysis of the secondary structure of CIPK protein showed that the protein encoded by the CIPK gene family of grape was mainly 伪 -helix and irregular curl, but the 尾 -turn angle was the least. After subcellular localization, VvCIPK gene was found to be the most expressed in the cytoplasm. The analysis of cis-acting elements in the 2kb region upstream of the CIPK gene family showed that VvCIPK13 had the most obvious response to ABA and dehydration stress, and the CIPK gene family of grape had a response to both MYB transcription factors and WRKY transcription factors. Fluorescence quantitative analysis showed that VvCIPK15 was the most expressed in root and the least in stem, and the difference of VvCIPK15 expression was significant under different treatments. Among them, the expression of PEGN ABA was up-regulated by NaCl, and the order of expression was PEGNaClABA. At the same time, the expression of the gene was up-regulated under high and low temperature stress, and only in sorbitum was down-regulated in 9 treatments. It is speculated that the gene may be involved in the regulation of drought, salinization, low temperature and other stress processes.
【作者单位】：甘肃农业大学园艺学院;
【基金】：甘肃农业大学国家级大学生科技创新项目(201510733005) 国家自然科学基金(31460500)~~
【分类号】：S663.1

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