新疆哈萨克民族CETP基因多态性与血脂及高脂血症中医证型关系

发布时间：2018-04-24 15:30

本文选题：哈萨克民族 + CETP基因　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:了解新疆哈萨克民族CETP基因A373P、c.*84GA突变位点多态性与血脂水平及高脂血症中医证型的关系。方法:方法:新疆乌鲁木齐周边地区作为调查点,采用随机整群抽样的方法,对哈萨克族居民聚集的社区、乡村等进行采样。按高脂血症流行病学调查方法制定调查表。抽血检测血清总胆固醇(TC)、甘油三(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。提取基因组DNA,用聚合酶链反应限制性片段长度多态性(PCR-RFLP)对218例新疆地区哈萨克族高脂血症患者和230例健康对照者进行CETP因分型,并对其血脂水平进行检测。对218例高脂血症患者进行中医辩证分型,比较其基因型与血脂水平及中医证型之间的关系。结果:448例受试者全部完成测试进入结果分析(1)共检出胆固醇酯转移蛋白的2个位点3种基因型AA、AG、GG,以GG基因型频率最高,新疆哈萨克民族高脂血症组中rs5880(c.*84GA)位点AA,AG和GG基因型频率分别为0例,11例和206例,高脂血症组中A和G的等位基因频率分别为2.5%和97.5%,健康对照组中A和G的等位基因频率分别为3.7%和96.3%。(2)新疆哈萨克民族CETP基因A373P基因型高脂血症组和健康对照组之间血浆TC,LDL-C有差异。rs1801706位点高脂血症中G等位基因携带者(AG、GG基因型)较非G等位基因携带者(AA基因型)有更高的血浆TC和LDL-C水平(P0.05);(3)rs1801706位点GG基因型中痰浊阻遏证最多有71例;其次为肝肾阴虚和脾肾阳虚占该基因型20.1%和15.3%。AG基因型中痰浊阻遏最多16例。AA基因型所有证型都很少,肝肾阴虚2例,痰浊阻遏1例。进一步统计学分析表明在GG、AA、AG基因型构成比中存在差异,痰浊阻遏高于肝肾阴虚,肝肾阴虚高于脾肾阳虚,脾肾阳虚高于气滞血瘀,气滞血瘀高于阴虚阳亢。结论:新疆地区转脂蛋白基因A373P、c.*84GA多态性与高脂血症水平可能不相关,A373P基因型GG与高脂血症痰瘀互阻可能相关。
[Abstract]:Objective: to investigate the relationship between the polymorphism of CETP gene A373Pnlc. 4GA mutation and hyperlipidemia and hyperlipidemia in Xinjiang Kazakh nationality. Methods: the area around Urumqi, Xinjiang, was sampled by random cluster sampling in the communities and villages where Kazakh residents gathered. The questionnaire was made according to the epidemiological investigation method of hyperlipidemia. Serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) were measured. Genomic DNAs were extracted and CETP typing was performed in 218 Kazak patients with hyperlipidemia and 230 healthy controls by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Two hundred and eighteen patients with hyperlipidemia were divided into two groups, and the relationship between genotype, blood lipid level and TCM syndromes was compared. Results all the 448 subjects completed the test and entered the analysis of the results. A total of 2 loci and 3 genotypes of cholesterol ester transfer protein (AAGG) were detected, with the highest frequency of GG genotypes. In Xinjiang Kazak ethnic hyperlipidemia group, the genotype frequencies of rs5880 and GG genotypes of AAAG and GG were 0 cases and 206 cases, respectively. The frequencies of A and G alleles in hyperlipidemia group were 2.5% and 97.5%, respectively, and the frequencies of A and G alleles in healthy control group were 3.7% and 96.33%, respectively.) Xinjiang Kazakh CETP gene A373P genotype hyperlipidemia group and healthy control group The plasma TC and LDL-C levels of G allele carriers of G allele in hyperlipidemia were significantly higher than those of non-G alleles carriers (AA genotype). There were 71 cases of turbid phlegm repressions in GG genotypes at GG locus 1801706. Secondly, deficiency of liver and kidney yin and deficiency of spleen and kidney yang accounted for 20.1% of the genotype. In the genotype of 15.3%.AG, there were 16 cases of phlegm turbid repress. All the syndrome types of AA genotype were few, liver and kidney yin deficiency in 2 cases, phlegm turbid repress in 1 case. Further statistical analysis showed that there were differences in the genotype composition ratio of GGG AA-AG, phlegm turbid repressor was higher than liver and kidney yin deficiency, liver and kidney yin deficiency was higher than spleen and kidney yang deficiency, spleen and kidney yang deficiency was higher than Qi stagnation and blood stasis, Qi stagnation and blood stasis was higher than yin deficiency and yang hyperactivity. Conclusion: there may be no correlation between the polymorphism of translipoprotein gene A373P and the level of hyperlipidemia in Xinjiang area. The GG genotype of A373P may be associated with phlegm and blood stasis resistance of hyperlipidemia.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R259

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