桑叶对糖尿病小鼠肝脏Toll样受体基因表达的影响

发布时间：2018-04-24 18:03

本文选题：糖尿病 + 桑叶水提物　；参考：《中国实验方剂学杂志》2017年06期

【摘要】：目的:探讨桑叶水提物对糖尿病小鼠肝组织Toll样受体(Toll-like receptors,TLRs)基因表达量的影响,并探讨其相关的作用机制。方法:ICR小鼠随机分为正常组(6只),糖尿病组。糖尿病组小鼠利用高脂高糖饲料喂养10周联合多次注射链脲佐菌素(STZ)建立高脂饲养和STZ共同诱导的小鼠糖尿病模型。造模成功小鼠随机分为模型组,二甲双胍组(600 mg·kg~(-1)),桑叶高、中、低剂量组(8,4,2 g·kg~(-1)),每组6只,灌胃(ig)给药,每周测空腹血糖,于实验中后期行口服糖耐量实验,ig给药10周后,测定小鼠血清胰岛素、总胆固醇、甘油三酯和肿瘤坏死因子-α(TNF-α),并应用实时荧光定量PCR(Real-time PCR)检测小鼠肝组织中TLR1~9表达量。结果:糖尿病模型小鼠经桑叶水提物治疗后,进水量减少,显著降低空腹血糖,改善糖耐量,显著降低血脂,增加胰岛素含量,改善胰岛素抵抗,同时降低血清中TNF-α(P0.05,P0.01)。Realtime PCR结果显示,糖尿病小鼠肝脏组织TLRs受体中TLR1,TLR2,TLR3,TLR7,TLR8和TLR9表达明显高于正常组(P0.05,P0.01),TLR5表达明显低于正常组;桑叶给药组后,桑叶中剂量组肝组织中的TLR7,TLR8和TLR9表达量,显著降低于模型组(P0.05,P0.01)。结论:桑叶水提物的降糖作用可能与抑制糖尿病模型小鼠肝组织中TLRs受体有关。
[Abstract]:Aim: to investigate the effect of mulberry leaf water extract on the expression of Toll-like receptor TLRs gene in liver tissue of diabetic mice and its related mechanism. Methods: ICR mice were randomly divided into normal group (n = 6) and diabetes group (n = 6). Diabetic mice were fed with high fat and high sugar diet for 10 weeks and were injected with streptozotocin (STZ) for 10 weeks to establish the diabetic model induced by hyperlipidemia and STZ. The successful mice were randomly divided into two groups: metformin group (600 mg / kg), mulberry leaf high, middle and low dose group (n = 6, n = 6, each group, n = 6). Fasting blood glucose was measured weekly, and oral glucose tolerance test was given 10 weeks later. Serum insulin, total cholesterol, triglyceride and tumor necrosis factor- 伪 TNF- 伪 were measured. The expression of TLR1~9 in liver tissue was measured by real-time fluorescence quantitative PCR(Real-time. Results: after treated with water extract from mulberry leaves, the intake of water decreased, fasting blood glucose decreased, glucose tolerance improved, blood lipids decreased, insulin content increased and insulin resistance improved. The expression of TLR7TLR8 and TLR7TLR8 in liver tissue of diabetic mice was significantly higher than that of normal group, and the expression of TLR7TLR8 and TLR9 in liver tissue of mulberry leaf medium dose group was significantly lower than that of normal control group, and the expression of TLR7TLR8 and TLR9 in liver tissue of mulberry leaf medium dose group was significantly lower than that of normal group after the administration of mulberry leaf, the expression of TLR7TLR8 and TLR9 in liver tissue of mulberry leaf treated group was significantly lower than that of normal control group, and the expression of TLR7TLR8 and TLR8 in liver tissue of mulberry leaf medium dose group was significantly lower than that of normal group. It was significantly lower than that of the model group (P 0.05, P 0.01). Conclusion: the hypoglycemic effect of mulberry leaf water extract may be related to the inhibition of TLRs receptor in liver tissue of diabetic mice.
【作者单位】：北京中医药大学中药学院;北京中医药大学北京中医药研究院;
【基金】：国家自然科学基金项目(81374027)
【分类号】：R285.5

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