巨疖蝙蛾不同虫态的COI基因条形码识别

发布时间：2018-04-25 00:32

  本文选题：巨疖蝙蛾 + DNA条形码　； 参考：《昆虫学报》2017年06期

【摘要】：【目的】巨疖蝙蛾Endoclita davidi(Poujade,1886)是雪峰虫草菌Ophiocordyceps xuefengensis的重要寄主。本研究分析了该虫线粒体DNA COI基因条形码,以在不同虫态及被虫草菌寄生的僵虫状态下能快速准确鉴定寄主种类。【方法】PCR扩增巨疖蝙蛾成虫(足一对,胸肌,翅基部)、卵(单粒)、幼虫(胸足一对)、蛹(少量体壁组织)及虫草僵虫(胸足或体壁)组织共计21个样品的COI基因片段(约658 bp)并进行测序和比对;以MEGA6.0软件的K2-P双参数模型计算种内及种间遗传距离;以邻接法(neighbor-joining,NJ)和最大简约法(maximum parsimony,MP)两种方法构建系统发育树。【结果】聚类分析与形态学鉴定结果一致,所纳入分析的20个样品均为同一物种,节点支持率可达99%。种内遗传距离为0.00%~1.08%,平均0.54%。与蝙蝠蛾科其他6种昆虫种间遗传距离为5.36%~13.31%,平均10.47%;种间遗传距离为种内遗传距离的19.39倍(10.47%vs 0.54%),而且种内与种间遗传距离没有重叠区域。【结论】基于mtDNA COI基因DNA条形码技术可以用于巨疖蝙蛾的快速准确鉴定,并对蝙蝠蛾科(Hepialidae)昆虫的分子系统发育分析有重要意义。
[Abstract]:[objective] Endoclita davidii Poujadea 1886 is an important host of Ophiocordyceps xuefengensis. In this study, the mitochondrial DNA COI gene bar code was analyzed, so that the host species could be identified quickly and accurately under different insect and parasitic conditions of Cordyceps. [methods] PCR was used to amplify adult adults (foot pair, pectoralis muscle) of Batacia megacephala (foot pair, pectoralis muscle). The COI gene fragments (about 658 BP) of 21 samples were obtained from the base of wings, eggs, larva (sternopoda pair), pupa (a small amount of body wall tissue) and Metarhizium anisopliae (chest foot or body wall). Phylogenetic tree was constructed by using K2-P two-parameter model of MEGA6.0 software and genetic distance between species and neighbor-joiningling NJs and maximum parsimony MP). [results] the results of cluster analysis and morphological identification were consistent. The 20 samples included in the analysis are all the same species, and the node support rate can reach 9910%. The intraspecific genetic distance was 0.001.08, with an average of 0.54. The genetic distance between species and other 6 species was 5.36, 13.31, with an average of 10.47, and the genetic distance between species was 19.39 times as high as that between species and 0.54.The genetic distance between species and species did not overlap. [conclusion] based on mtDNA COI gene DNA, the interspecific genetic distance was 19.39 times higher than that of other 6 species. [conclusion] there was no overlap between the interspecific genetic distance and the interspecific genetic distance. [conclusion] The form-coding technique can be used for rapid and accurate identification of Phasachis macrophylla. It is important to analyze the molecular phylogeny of Hepialidaeae.
【作者单位】： 湖南师范大学生命科学学院;
【基金】：国家自然科学基金项目(31672094) 湖南省科技厅重大专项(2014FJ1007) 湖南省生态学重点学科建设项目(0713)
【分类号】：Q963
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本文编号：1798983