月季抗白粉病基因RhMLO的亚细胞定位及功能分析

发布时间：2018-04-25 03:06

本文选题：月季 + 白粉病　；参考：《园艺学报》2017年05期

【摘要】：MLO是一类新发现的抗病基因,一些双子叶植物隐性突变的mlo基因使其获得了广谱高抗的抗病性。通过生物信息学分析结合亚细胞定位试验,对前期克隆获得的月季MLO进行亚细胞定位,结果表明,RhMLO1和RhMLO2均为主要分布于细胞质膜、液泡膜和细胞核的跨膜蛋白,与预测结果相符。构建了RhMLO1的正义表达载体,通过农杆菌介导法对月季‘白玉’植株体细胞胚进行遗传转化。利用PCR法和荧光定量PCR法对转基因植株进行分子检测,表明基因已经整合到转基因植株中。分别利用离体鉴定法和显微镜观察法对转基因植株和对照植株进行对白粉病菌(Podosphera pannosa)抗性鉴定,结果一致显示:正义载体的导入降低了转基因植株对白粉病的抗性。
[Abstract]:MLO is a newly discovered disease resistant gene, and the MLO gene of some dicotyledonous recessive mutations makes it obtain broad spectrum and high resistance to disease. By bioinformatics analysis and subcellular localization test, the subcellular localization of MLO obtained from the prophase clone is carried out. The results show that both RhMLO1 and RhMLO2 are mainly distributed in cell plasmalemma and liquid. The transmembrane proteins of the membrane and the nucleus were consistent with the predicted results. A just expression vector of RhMLO1 was constructed, and the somatic embryos of the white jade plant were transformed by Agrobacterium tumefaciens. The transgenic plants were detected by PCR method and fluorescence quantitative PCR method, and the gene had been integrated into the transgenic plants. The resistance of transgenic plants and control plants to Podosphera pannosa was identified by the method of isolation and microscope observation. The results showed that the introduction of justice carrier reduced the resistance of transgenic plants to powdery mildew.

【作者单位】：云南省农业科学院花卉研究所国家观赏园艺工程技术研究中心云南省花卉育种重点实验室;
【基金】：国家自然科学基金项目(31560565;31160403) 云南省应用基础研究计划项目(2014FB158) 云南省中青年学术技术带头人后备人才培养项目(2015HB078) 国家科技支撑计划项目(2015BAD10B01)
【分类号】：S685.12

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