绵果荠CBF基因启动子克隆及诱导特性分析

发布时间：2018-04-25 04:33

本文选题：绵果荠 + LlCBF　；参考：《分子植物育种》2017年05期

【摘要】：CBF基因不仅是植物CBF抗冷途径的枢纽,而且可以提高植物的抗冷能力。而植物的抗冷能力的提高是由于CBF基因调控下游抗冷基因的表达来实现的。本研究根据前期已克隆得到的绵果荠Ll CBF基因(JQ687132)序列,利用TAIL-PCR方法从新疆早春短命植物绵果荠基因组DNA中分离得到Ll CBF基因编码区上游1 172 bp启动子序列;将该序列连入克隆载体并测序,通过生物信息学分析表明,该序列含TATAbox、CAAT-box等典型的真核生物核心启动子元件,同时具有等逆境响应相关元件。在烟草叶片中的瞬时表达分析,进一步表明,在低温、干旱及盐胁迫诱导下该启动子序列具有驱动GUS报告基因表达的特性。本研究对新疆早春短命植物种质资源的开发利用及丰富作物逆境基因工程诱导启动子资源具有重要的实践意义。
[Abstract]:CBF gene is not only the hinge of plant CBF cold resistance pathway, but also can improve the cold resistance ability of plants. The improvement of plant cold resistance is due to the regulation of downstream cold resistance gene expression by CBF gene. Based on the previously cloned Ll CBF gene JQ687132) sequence, 1 172 BP promoter sequence was obtained from the genomic DNA of Cardamine cardamine, a short-lived plant in early spring in Xinjiang, by TAIL-PCR method. The sequence was inserted into the clone vector and sequenced. Bioinformatics analysis showed that the sequence contained typical eukaryote core promoter elements such as TATAbox and CAAT-box. The transient expression analysis in tobacco leaves further showed that the promoter sequence had the characteristics of driving the expression of GUS reporter gene under the induction of low temperature, drought and salt stress. This study is of great practical significance for the development and utilization of germplasm resources of short-lived plants in early spring in Xinjiang and the enrichment of genetic engineering induced promoter resources for crop stress.
【作者单位】：新疆农业大学农业生物技术重点实验室;
【基金】：国家大学生创新创业训练计划项目(201400758002)资助
【分类号】：Q943.2

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