青海地区藏汉民族子痫前期与HLA-G基因非编码区多态性的相关性研究

发布时间：2018-04-25 09:45

本文选题：子痫前期 + 人类白细胞抗原G　；参考：《青海大学》2017年硕士论文

【摘要】：目的:通过研究青海地区藏汉民族子痫前期患者与HLA-G基因非编码区多态性的相关性,从而筛选出青海地区藏汉民族孕妇易感子痫前期所对应的HLA-G多态性位点,为子痫前期的病因学研究提供依据,指导临床对子痫前期的预防。方法:收集2015年4月到2016年10月期间在青海大学附属医院产科分娩的藏汉民族正常妊娠孕妇及子痫前期患者的血液标本共348例,采用DNA提取、聚合酶联反应技术、琼脂糖凝胶电泳及DNA测序等方法找出HLA-G基因非编码区存在的多态性位点,进而通过相应的统计学方法分析四组间各位点的等位基因及基因型分布是否存在差异,最后得出相应的结论。结果:四组间HLA-G的Exon8 14bp插入/缺失多态性分布差异无统计学意义,而将基因型合并后发现II基因型和ID/DD基因型分布在汉族正常妊娠组和藏族正常妊娠组之间差异具有统计学意义;在HLA-G的5-URR区域发现6个单核苷酸多态性位点,其中2个(-289、-274)位点多态性在四组间分布差异具有统计学意义,两两比较后-289位点的等位基因分布在汉族正常妊娠组和汉族子痫前期组之间有统计学差异,-274位点的基因型和等位基因在汉族正常妊娠组和藏族正常妊娠组之间有统计学差异,-289/-274位点联合基因型在汉族正常妊娠组和汉族子痫前期组之间有统计学差异。结论:HLA-G基因Exon8 14bp插入/缺失位点多态性和HLA-G基因5-URR的-274位点多态性可能与民族差异有关,而与青海地区藏汉族孕妇子痫前期的易感性的关系还需进一步扩大样本数量来探讨;HLA-G基因5-URR的-289位点多态性可能与青海地区汉族子痫前期的易感性有关;HLA-G基因5-URR的-289/-274位点联合多态性可能与青海地区汉族子痫前期的易感性有关。
[Abstract]:Objective: to study the association between preeclampsia and non-coding region polymorphism of HLA-G gene in Tibetan and Han nationality preeclampsia patients in Qinghai area, and to screen out the HLA-G polymorphism corresponding to preeclampsia in Tibetan and Han nationality pregnant women in Qinghai area. To provide the basis for the etiological study of preeclampsia and to guide the clinical prevention of preeclampsia. Methods: from April 2015 to October 2016, the blood samples of normal pregnant women and preeclampsia were collected from 348 pregnant women and preeclampsia patients who were delivered by obstetrics in the affiliated Hospital of Qinghai University. DNA extraction and polymerase chain reaction (PCR) were used. Agarose gel electrophoresis and DNA sequencing were used to identify the polymorphic loci in the non-coding region of the HLA-G gene, and then the alleles and genotypes of the four groups were analyzed by statistical methods. Finally, the corresponding conclusions are drawn. Results: there was no significant difference in the distribution of Exon8 14bp insertion / deletion polymorphism in HLA-G among the four groups. It was found that the distribution of genotype II and ID/DD genotype was statistically significant between normal pregnancy group of Han nationality and normal pregnancy group of Tibetan nationality, and six single nucleotide polymorphism loci were found in 5-URR region of HLA-G. There were significant differences in the distribution of two loci polymorphism among the four groups. The allele distribution of -289 locus was significantly different between normal pregnancy group and preeclampsia group of Han nationality. The genotype and allele of -274 locus were found in normal pregnancy group of Han nationality and normal pregnancy group of Tibetan nationality. Statistical difference was found between Han normal pregnancy group and Han preeclampsia group. Conclusion the polymorphism of insertion / deletion locus of Exon8 14bp of HLA-G gene and the polymorphism of 5-URR locus of HLA-G gene may be related to ethnic differences. The relationship between preeclampsia and preeclampsia in Tibetan and Han women in Qinghai area needs to be further expanded to explore the relationship between HLA-G gene 5-URR polymorphism and preeclampsia preeclampsia preeclampsia (5-URR) gene in Qinghai Han nationality. The-289%-274 locus polymorphism may be associated with preeclampsia susceptibility in the Han nationality in Qinghai.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.244

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