从江香猪LMF1基因的克

发布时间：2018-04-25 10:23

本文选题：从江香猪 + LMF　；参考：《西南大学学报(自然科学版)》2017年12期

【摘要】：为研究脂肪酶成熟因子1(LMF1)基因在从江香猪不同组织的表达情况及在真核细胞中的亚细胞定位情况,采用RT-PCR,Real-Time PCR,PSORT II Prediction软件结合荧光共定位的方法,克隆了从江香猪LMF1基因cDNA,构建了pEGFP-C1-LMF1真核表达载体,分析了LMF1基因在不同组织的表达差异和亚细胞定位情况.结果表明,从江香猪LMF1基因与GenBank上提交的其他猪种的同源性达到了100%;LMF1基因在脂肪中表达量最高,心中最低,且脂肪和小肠与其他7个组织相比,表达量均存在统计学意义(p0.01);荧光共定位结果表明,LMF1基因主要集中在细胞质中表达.相关结果对后续研究LMF1蛋白与其互作蛋白在脂质代谢的作用机理奠定的基础.
[Abstract]:In order to study the expression of lipase maturation factor 1 (LMF1) gene in different tissues of Congjiang Xiang pig and its subcellular localization in eukaryotic cells, RT-PCRX Real-Time PCRN- PSORT II Prediction software and fluorescence co-localization method were used to study the expression of lipase maturation factor 1 (LMF1) gene in different tissues of Congjiang Xiang pig. The LMF1 gene of Congjiang Xiang pig was cloned and the eukaryotic expression vector of pEGFP-C1-LMF1 was constructed. The difference of expression and subcellular localization of LMF1 gene in different tissues was analyzed. The results showed that the homology of LMF1 gene of Congjiang Xiang pig and other pig breeds submitted on GenBank was the highest in fat and the lowest in heart, and the fat and small intestine were compared with other 7 tissues. The results of fluorescence co-localization showed that the LMF1 gene was mainly expressed in the cytoplasm. The results laid a foundation for further study on the mechanism of LMF1 protein and its interaction protein in lipid metabolism.
【作者单位】：贵州大学高原山地动物遗传育种与繁殖教育部重点实验室;贵州大学动物科学学院;贵州露元现代农业发展有限公司;
【基金】：省校联合基金项目(黔科合LH字[2014]7668) 贵州大学青年基金项目(贵大自青基合字[2012]010号)
【分类号】：Q953

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