刺芹侧耳多肽对染铅大鼠脑海马组织形态及海马组织中camk2a、erk2和homer1基因mRNA表达量的影响

发布时间：2018-04-26 11:47

本文选题：刺芹侧耳多肽 + N-甲基-D-天冬氨酸受体　；参考：《食用菌学报》2017年02期

【摘要】：为研究刺芹侧耳(Pleurotus eryngii)多肽对染铅大鼠脑组织损伤的影响,以雄性SD大鼠为研究对象,随机分为正常对照组(基础饲料,蒸馏水)、染铅模型组(基础饲料,0.2%醋酸铅水溶液)和多肽组(添加2%多肽的基础饲料,0.2%醋酸铅水溶液),饲养60d后,观察大鼠脑部海马组织形态变化,测定海马组织中铅含量、camk2a、erk2和homer1mRNA的表达量。结果表明:铅会导致海马组织的细胞数量减少、细胞层变薄、细胞间隙增大、camk2a、erk2和homer1mRNA表达量极显著降低;与染铅模型组相比,多肽组海马组织的铅含量显著降低、细胞数目增多、细胞核变大、细胞间隙缩小,同时海马组织中NMDAR和mGluR5信号通路相关基因camk2a、erk2和homer1mRNA的表达量显著或极显著升高,表明刺芹侧耳多肽可减轻铅对大鼠海马组织的损伤。
[Abstract]:In order to study the effect of Pleurotus eryngii polypeptide on brain tissue injury in lead exposed rats, male SD rats were randomly divided into normal control group (basal diet). Distilled water, lead exposed model group (basic diet 0.2% lead acetate aqueous solution) and polypeptide group (0.2% lead acetate solution supplemented with 2% polypeptide) were fed for 60 days, and the morphological changes of hippocampal tissue were observed. The content of lead in hippocampus and the expression of camk2a erk2 and homer1mRNA were measured. The results showed that lead could decrease the number of hippocampal cells, thin the cell layer, decrease the expression of camk2aerk2 and homer1mRNA, and decrease the content of lead and increase the number of cells in polypeptide group compared with the lead exposed model group. The expression of NMDAR and mGluR5 signaling pathway related genes camk2aerk2 and homer1mRNA were significantly or extremely increased in hippocampus, which indicated that polypeptide of Pleurotus prickly could attenuate the damage caused by lead in hippocampus of rats.
【作者单位】：山西农业大学食品科学与工程学院;山西省食用菌工程技术研究中心;
【基金】：山西省重点研发计划项目(201603D2011201) 山西省科技攻关项目(20130311017-3) 山西省煤基重点科技攻关项目(FT2014-03-01) 黄土高原食用菌提质增效协同创新中心资助
【分类号】：R285.5

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