外源油菜素内酯对弱光下番茄幼苗光合碳同化关键酶及其基因的影响

发布时间：2018-04-26 22:37

本文选题：番茄 + 弱光胁迫　；参考：《园艺学报》2016年10期

【摘要】：以弱光敏感型番茄品种‘基尔斯’为试验材料,研究叶面喷施24 表油菜素内酯(EBR)对弱光胁迫下番茄幼苗生长、光合碳同化关键酶活性及基因表达的影响。结果表明,弱光胁迫下幼苗质量、茎粗和壮苗指数分别下降了70.5%、13.0%和77.7%,而株高增加了45.3%,说明外源EBR显著缓解了弱光胁迫对幼苗生长的抑制。弱光胁迫使番茄叶片净光合速率(Pn)降低42.8%,而喷施EBR后Pn提高到对照的72.0%。弱光胁迫导致番茄光合碳同化相关的核酮糖 1,5 二磷酸羧化/加氧酶(Rubisco)、果糖 1,6 二磷酸醛缩酶(Ald)、果糖 1,6 二磷酸酯酶(FBPase)和Rubisco活化酶(RCA)活性分别降低41.6%、31.3%、31.4%和19.6%,EBR处理后显著提高了这些酶的活性。同时弱光胁迫显著降低碳同化关键酶基因Rbc L、Rbc S、RCA、Ald、FBPase、SBPase、GAPDH、TK和PRK的表达量,而EBR显著提高这些基因的表达水平,其中SBPase和GAPDH的m RNA转录接近对照水平,TK和PRK的m RNA转录水平比对照提高了29%和37%。这些结果表明外源EBR通过调控弱光胁迫下番茄幼苗碳同化相关酶活性及其基因表达,提高叶片净光合速率,缓解弱光胁迫对植株的伤害,从而增强番茄幼苗的弱光耐性。
[Abstract]:The effects of 24 epibrassinolide (EBR) spraying on the growth, photosynthetic carbon assimilation key enzyme activity and gene expression of tomato seedlings under low light stress were studied. The results showed that seedling quality, stem diameter and seedling strength index decreased by 70.5% and 77.7%, respectively, while plant height increased by 45.3% under low light stress, indicating that exogenous EBR significantly alleviated the inhibition of seedling growth under low light stress. The net photosynthetic rate (PN) of tomato leaves decreased by 42.8% under low light stress, while PN increased to 72.0% of the control after spraying EBR. The activities of ribonose carboxylation / oxygenase associated with photosynthesis carbon assimilation in tomato, Rubiscotor, fructose 1, diphosphate aldolase, fructose 1, diphosphatase (FB Pase2) and Rubisco activator (rca) were reduced by 41.631. 3% and 19. 6%, respectively, in tomato photosynthesis under low light stress, and decreased by 31. 4% and 19. 6% respectively. The activity of these enzymes was significantly increased after treatment. At the same time, low light stress significantly reduced the expression of the key enzyme gene Rbc Lbc Rbc RCAA Aldfb Pasesii SBAP DHN TK and PRK, while EBR significantly increased the expression level of these genes. The m RNA transcription of SBPase and GAPDH was similar to that of the control level. The m RNA transcription level of TK and PRK was increased by 29% and 37% than that of the control. These results indicated that exogenous EBR could increase the net photosynthetic rate of leaves and alleviate the injury of tomato seedlings under low light stress by regulating the activity of carbon assimilation related enzymes and gene expression of tomato seedlings under low light stress, thus enhancing the low light tolerance of tomato seedlings.
【作者单位】：南京农业大学园艺学院;
【基金】：国家现代农业产业技术体系建设专项资金项目(CARS-25-C-03) 江苏省农业三新工程项目(SXGC[2014]256,SXGC[2015]321,SXGC[2016]177)
【分类号】：S641.2

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