湖羊NR5A1基因SNPs筛选及其与产羔数的关联分析

发布时间：2018-04-28 15:34

  本文选题：湖羊 + NRA基因　； 参考：《江苏农业学报》2017年01期

【摘要】：为探讨NR5A1基因能否作为湖羊产羔性状的候选基因,同时寻找与湖羊繁殖性能相关的分子标记,以高繁殖力湖羊为研究对象,采用DNA池结合测序方法筛选NR5A1基因在湖羊中的SNPs位点,并利用PCR-RFLP和AS-PCR方法进行多态位点分型,利用SPSS软件分析不同基因型与湖羊产羔数的相关性。结果表明,在湖羊NR5A1基因中检测到6个SNPs位点,分析发现g.6052A/G位点为Taq I酶切位点,其余5个位点处没有酶切位点存在。在湖羊群体中,g.3362G/C(GG、GC、CC)、g.4342G/A(GG、GA、AA)、g.4666C/T(CC、CT、TT)、g.6052A/G(GG、AG、AA)和g.6991T/G(TT、TG、GG)位点均检测到3种基因型,而g.5699C/G位点在湖羊群体中仅发现2种基因型GG和GC。6个位点的多态信息含量(PIC)从0.306到0.375不等,均属于中度多态(0.25PIC0.50),杂合度(He)从0.377到0.500,在这6个SNPs位点中,仅g.6052A/G位点处于Hardy-Weinberg平衡状态。连锁分析发现,g.3362G/C和g.6052A/G 2位点在湖羊群体中紧密连锁,二者共构建了8种单倍型组合,AAGG为主要的单倍型,占总数的35.90%,AGCG和AACG次之,分别占总数的20.30%和18.75%。关联分析发现g.6052A/G位点GG型湖羊的平均产羔数显著高于AA型(P0.05)和AG型(P0.05),其余位点的各基因型在湖羊的头胎产羔数、二胎产羔数、三胎产羔数和平均产羔数间差异均不显著。AAGG单倍型和AACG单倍型的二胎产羔数比AGCG单倍型的二胎产羔数分别多0.40(P0.05)和0.53(P0.01),差异显著。说明,NR5A1基因对湖羊产羔数有一定的影响,g.6052A/G位点、g.3362 G/C和g.6052A/G连锁可作为湖羊繁殖性能的有效遗传标记,用于湖羊的分子选育。
[Abstract]:In order to investigate whether NR5A1 gene can be used as candidate gene for lambing traits of Hu sheep, and to search for molecular markers related to reproduction performance of Hu sheep, the high fecundity Hu sheep was used as the research object. The SNPs loci of NR5A1 gene in Hu sheep were screened by DNA pool and sequencing. The polymorphic loci were classified by PCR-RFLP and AS-PCR. The correlation between different genotypes and litter size of Hu sheep was analyzed by SPSS software. The results showed that six SNPs loci were detected in the NR5A1 gene of Hu sheep. The g.6052A/G loci were found to be Taq I digested sites, while the other 5 loci were not found. In the Hu sheep population, three genotypes were detected, and the polymorphic information contents of the two genotypes (GG and GC.6 loci) were found in the Hu sheep population. The polymorphic information content of the two genotypes (GG and GC.6) ranged from 0.306 to 0.375, and the polymorphic information contents of the GG and GC.6 loci were only found in the Hu sheep population. All of them belong to 0.25PIC0.50, and the heterozygosity is from 0.377 to 0.500.The only g.6052A/G loci in the six SNPs loci are in the Hardy-Weinberg equilibrium state. Linkage analysis showed that the loci of G. 3362G / C and g.6052A/G 2 were closely linked in Hu sheep population. Eight haplotypes were constructed, accounting for 35.90% and 18.75% of the total, respectively. Correlation analysis showed that the average litter size of GG type Hu sheep at g.6052A/G locus was significantly higher than that of AA type P 0.05) and AG type P 0.05%. The other genotypes were in the first litter size and second litter size of Hu sheep. There was no significant difference in the number of lambs and the average number of lambs. The second litter size of the haplotypes of AAGG and AACG haplotypes was 0.40g / P0.05 and 0.53g / 0.01respectively more than that of AGCG haplotypes, and the difference was significant. These results suggest that the linkage of NR5A1 gene to litter size of Hu sheep can be used as an effective genetic marker for breeding performance of Hu sheep and can be used in molecular breeding of Hu sheep. The linkage of G. 6052A / G / G locus and g.6052A/G can be used as an effective genetic marker for breeding performance of Hu sheep.
【作者单位】： 江苏省农业科学院畜牧研究所;江苏省农业科学院动物品种改良和繁育重点实验室;
【基金】：江苏省农业科技自主创新基金项目[CX(14)5031] 江苏省自然科学基金项目(BK20140750) 国家自然科学基金项目(31501934)
【分类号】：S826
，

本文编号：1815867