沉默生物钟基因PER1对口腔鳞状细胞癌细胞中生物钟基因网络的影响

发布时间：2018-04-28 20:41

本文选题：生物钟 + 鳞状细胞癌　；参考：《华西口腔医学杂志》2017年01期

【摘要】：目的探讨口腔鳞状细胞癌细胞中生物钟基因PER1对生物钟基因网络中其他生物钟基因表达的影响。方法采用RNA干扰技术沉默人口腔鳞状细胞癌SCC15细胞中PER1基因,应用流式细胞仪检测沉默后细胞的增殖和凋亡水平,实时荧光定量聚合酶链式反应检测生物钟基因CLOCK、BMAL1、PER1、PER2、PER3、DEC1、DEC2、CRY1、CRY2、TIM、CKIE、RORA、NPAS2、REV-ERBA m RNA的表达情况。结果 PER1基因沉默后,SCC15细胞增殖指数上升,凋亡指数下降(P0.05);PER1、PER2、DEC1、DEC2、CRY1、CRY2和NPAS2 m RNA的表达水平降低(P0.05),PER3、TIM、RORA和REV-ERBA m RNA的表达水平升高(P0.05),CLOCK、BMAL1和CKIE m RNA的表达水平无统计学改变(P0.05)。结论生物钟基因PER1能够调控生物钟基因网络中众多其他生物钟基因PER2、DEC1、DEC2、CRY1、CRY2、NPAS2、PER3、TIM、RORA和REV-ERBA的表达,PER1在生物钟基因网络中具有重要调控作用。
[Abstract]:Objective to investigate the effect of clock gene PER1 on the expression of other clock genes in oral squamous cell carcinoma cells. Methods RNA interference technique was used to silence PER1 gene in human oral squamous cell carcinoma (SCC15) cells. Flow cytometry was used to detect the proliferation and apoptosis of the cells. Real-time quantitative polymerase chain reaction (FQ-PCR) was used to detect the expression of clock gene BMAL1, PER2PER3, DEC2, CRY1, CRY2, CRY2, CRY2, TIMY2, CRY2, TIMY2, and NPAS2, REV-ERBA m RNA. Results after PER1 gene silencing, the proliferation index of SCC15 cells increased, while the apoptotic index decreased. The expression levels of CRY2 and NPAS2 m RNA in P0.05 PER1PER2PER2DEC2DEC2DEC2 and NPAS2 m RNA decreased, and the expression levels of P0.05PER3TIMM-RORA and REV-ERBA m RNA increased, and the expression levels of P0.05CK-BMAL1 and CKIE m RNA did not change significantly (P 0.05). Conclusion the expression of clock gene PER1 can regulate the expression of clock gene PER2DEC1, CRY1, CRY2, NPAS2PER3, TIMRORA and REV-ERBA in the biological clock gene network. PER1 plays an important role in regulating the expression of PER1 in the biological clock gene network.
【作者单位】：重庆医科大学附属第一医院口腔颌面外科;
【分类号】：R739.8

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