KCNE1基因G38S多态性与新疆维吾尔族慢性心力衰竭的相关性研究

发布时间：2018-04-30 07:30

本文选题：慢性心力衰竭 + 基因型　；参考：《石河子大学》2017年硕士论文

【摘要】：目的:通过对KCNE1基因G38S基因分型,统计分析新疆维吾尔族射血分数减少型慢性心力衰竭患者(病例组)与对照组(无慢性心力衰竭患者)AA、AG、GG基因型及等位基因A、G频率分布。简述KCNE1基因G38S多态性与维吾尔族慢性心力衰竭的相关性。方法:选取2014年12月至2016年7月新疆维吾尔自治区人民医院就诊的维吾尔族患者中病例组200例,对照组200例。分别对两组进行基因分型,MSPA1酶切检测。结果:KCNE1基因G38S AA、AG、GG基因型人数在病例组为24(12.0%)、89(44.5%)、87(43.5%),对照组分别为37(18.5%)、91(45.5%)、72(36.0%)。等位基因A、G病例组为137(34.25%),263(65.75%),对照组为165(41.25%),235(58.75%)。病例组和对照组均符合Hardy-Weinberg平衡检验,具有群体代表性,P值均0.05。病例组和对照组中AG基因型较AA型和GG型增多,但统计学分析两组之间基因型比较无统计学差异(χ2=4.208,P=0.122),等位基因频率两组比较有统计学意义(χ2=4.170,P=0.041)。两组内男女基因型及等位基因频率统计分析无统计学意义(P0.05)。经logistic回归模型显示左室舒张末内径的OR值为1.473,β=0.387,95%CI:(1.357~1.599),QRS时限OR值为1.028,β=0.027,95%CI:(1.009~1.047),性别的OR值为2.288,β=0.828,95%CI:(1.059~4.943),冠心病的OR值为3.047,β=1.114,95%CI:(1.532~6.063),糖尿病OR值为3.200,β=1.163,95%CI:(1.345~7.562)。基因型AG的OR值为0.489,β=-0.715,95%CI:(0.247~0.966)。结论:1.维吾尔族慢性心力衰竭患者中KCNE1基因G38S携带G等位基因频率高于对照组(无慢性心力衰竭患者);2.左室舒张末径增加、QRS波时限延长、男性、冠心病、糖尿病均是慢性心力衰竭的危险因素,携带AG基因型发生慢性心力衰竭的风险小,基因型AG可能为发生慢性心力衰竭的保护因素,KCNE1基因G38S多态性与维吾尔族慢性心力衰竭具有相关性。
[Abstract]:Objective: to analyze the frequency distribution of KCNE1 gene GG genotype and allele in Xinjiang Uygur patients (case group) and control group (control group) by G38S genotyping of KCNE1 gene. The association between KCNE1 gene G38S polymorphism and Uygur chronic heart failure was briefly described. Methods: from December 2014 to July 2016, 200 cases of Uygur patients in Xinjiang Uygur Autonomous region people's Hospital and 200 cases of control group were selected. The genotyping of MSPA1 was detected in two groups. Results the GG genotypes of G38S AAAAGG of the KCNE1 gene were 240.12.0 and 898944.5in the case group. The GG genotypes of the G38S AAAGG gene of the KCNE1 gene were as follows: in the case group, the GG genotypes of the G38S AAAAGG gene were 240.12.0 and 898944.5in the case group, and in the control group the GG genotypes of the G38S AAAAGG gene were 37 ~ 18.5A / 45.5C, respectively. The alleles of allele Agna G in the case group were 137 / 34.25 / 263N / 65.75, and in the control group were 165 / 41.25 / 235 / 58.75g / L, respectively. Both the case group and the control group were in accordance with Hardy-Weinberg balance test, and the P value of group representative was 0.05. AG genotype was higher in case group and control group than that in AA type and GG type, but there was no significant difference in genotype between two groups (蠂 ~ 2 / 4.208). Allele frequency was significantly higher in two groups (蠂 ~ 2 / 4.170) (蠂 ~ (2) = 4.170) (P = 0.041). There was no significant difference in the frequency of genotype and allele between male and female in the two groups (P 0.05). The logistic regression model showed that the OR value of left ventricular end-diastolic diameter was 1.473, the OR value of 尾 0.38795 CIW 1.3571.599 QRS was 1.028, 尾 0.02795CIW 1.00995 CIW 1.0091.047m, the OR value of gender was 2.288, 尾 0.82895CIU 1.0594.943, the OR value of coronary heart disease was 3.047, 尾 1.11495 CICell 1.5326.06363, the OR value of diabetes was 3.200, 尾 1.16395CIU was 1.3457.562. The OR value of genotype AG was 0.489, and 尾 -0.715 + 95% CI: 0.247 ~ 0.966. Conclusion 1. The frequency of G allele of KCNE1 gene G38S in Uygur patients with chronic heart failure was higher than that in control group. Left ventricular end-diastolic diameter increased QRS wave duration. Men, coronary heart disease, diabetes mellitus were risk factors of chronic heart failure, and had a low risk of chronic heart failure with AG genotype. Genotype AG may be a protective factor for chronic heart failure. The G38S polymorphism of KCNE1 gene is associated with chronic heart failure in Uygur nationality.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.6

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