醌型二氢生物喋呤还原酶基因表达变化对高糖环境下NRK-52E细胞葡萄糖调节蛋白75表达的影响

发布时间：2018-04-30 04:39

  本文选题：糖尿病肾病 + 醌型二氢生物喋呤还原酶　； 参考：《中国糖尿病杂志》2017年04期

【摘要】：目的探讨醌型二氢生物喋呤还原酶(QDPR)基因对高糖环境下肾小管上皮细胞系NRK-52E葡萄糖调节蛋白75(GRP75)的影响及其在DN中的可能作用机制。方法构建过表达及敲低QDPR基因的NRK-52E模型,分别予5.4 mmol/L和30 mmol/L葡萄糖培养基培养,Western blot检测GRP75在OLETF大鼠肾皮质及细胞模型各组的表达水平,流式细胞术检测细胞周期。结果与LETO鼠比较,OLETF大鼠肾皮质GRP75含量降低[(1.53±0.27)vs(0.79±0.26),P0.01];与NRK-52E组比较,NRK-52E高糖组的GRP75含量降低[(0.62±0.03)vs(0.46±0.06)]、G0/G1期细胞增多[(39.80±1.31)%vs(50.35±0.33)%]、S期细胞减少[(48.55±1.85)%vs(37.17±0.10)%](P0.05);过表达QDPR使高糖环境下GRP75含量降低[(0.85±0.10)vs(0.45±0.16),P0.05]、G0/G1期细胞增多[(43.73±0.48)%vs(61.87±0.17)%,P0.01],S期细胞减少[(42.42±0.66)%vs(25.29±0.12)%,P0.01];敲低QDPR不影响GRP75表达量与细胞周期。结论 GRP75在DN模型中含量减少,QDPR基因可能通过GRP75及细胞周期影响DN发生发展。
[Abstract]:Objective to investigate the effect of quinone dihydrotrexate reductase (QDP) gene on renal tubular epithelial cell line NRK-52E glucose regulatory protein 75 GRP 75 and its possible mechanism in DN. Methods the NRK-52E model of overexpression and knockout of QDPR gene was constructed. The expression of GRP75 in the renal cortex and cell model of OLETF rats was detected by Western blot in 5.4 mmol/L and 30 mmol/L glucose culture medium, respectively. The cell cycle was detected by flow cytometry. 缁撴灉涓嶭ETO榧犳瘮杈，

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